Curated Information
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Curated Information Page
PubMed Id: 20048001 
Zhao B, et al. (2010) A coordinated phosphorylation by Lats and CK1 regulates YAP stability through SCF(beta-TRCP). Genes Dev 24, 72-85 20048001
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S61-p - YAP1 iso2 (human)
Orthologous residues
YAP1 (human): S61‑p, YAP1 iso2 (human): S61‑p, YAP1 iso3 (human): S61‑p, YAP1 (mouse): S46‑p, YAP1 (rat): S46‑p, YAP1 iso3 (rat): S46‑p, YAP1 (sheep):
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LATS1 (human) transfection of wild-type enzyme, mutation in upstream enzyme recognition motif

T63-p - YAP1 iso2 (human)
Orthologous residues
YAP1 (human): T63‑p, YAP1 iso2 (human): T63‑p, YAP1 iso3 (human): T63‑p, YAP1 (mouse): T48‑p, YAP1 (rat): T48‑p, YAP1 iso3 (rat): T48‑p, YAP1 (sheep):
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S109-p - YAP1 iso2 (human)
Orthologous residues
YAP1 (human): S109‑p, YAP1 iso2 (human): S109‑p, YAP1 iso3 (human): S109‑p, YAP1 (mouse): S94‑p, YAP1 (rat): S94‑p, YAP1 iso3 (rat): S94‑p, YAP1 (sheep): S24‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LATS1 (human) transfection of wild-type enzyme, mutation in upstream enzyme recognition motif

S127-p - YAP1 iso2 (human)
Orthologous residues
YAP1 (human): S127‑p, YAP1 iso2 (human): S127‑p, YAP1 iso3 (human): S127‑p, YAP1 (mouse): S112‑p, YAP1 (rat): S112‑p, YAP1 iso3 (rat): S112‑p, YAP1 (sheep): S42‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LATS1 (human) transfection of wild-type enzyme, mutation in upstream enzyme recognition motif
Downstream Regulation
 Effect of modification (function):  intracellular localization
 Effect of modification (process):  carcinogenesis, inhibited

S138-p - YAP1 iso2 (human)
Orthologous residues
YAP1 (human): S138‑p, YAP1 iso2 (human): S138‑p, YAP1 iso3 (human): S138‑p, YAP1 (mouse): S123‑p, YAP1 (rat): , YAP1 iso3 (rat): , YAP1 (sheep): S53‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S164-p - YAP1 iso2 (human)
Orthologous residues
YAP1 (human): S164‑p, YAP1 iso2 (human): S164‑p, YAP1 iso3 (human): S164‑p, YAP1 (mouse): S149‑p, YAP1 (rat): S146‑p, YAP1 iso3 (rat): S146‑p, YAP1 (sheep): S79‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LATS1 (human) transfection of wild-type enzyme, mutation in upstream enzyme recognition motif

S289-p - YAP1 iso2 (human)
Orthologous residues
YAP1 (human): S289‑p, YAP1 iso2 (human): S289‑p, YAP1 iso3 (human): S251‑p, YAP1 (mouse): S274‑p, YAP1 (rat): S271‑p, YAP1 iso3 (rat): S271‑p, YAP1 (sheep): S204‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S351-p - YAP1 iso2 (human)
Orthologous residues
YAP1 (human): S367‑p, YAP1 iso2 (human): S351‑p, YAP1 iso3 (human): S317‑p, YAP1 (mouse): S352‑p, YAP1 (rat): S333‑p, YAP1 iso3 (rat): , YAP1 (sheep): S266‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S381-p - YAP1 iso2 (human)
Orthologous residues
YAP1 (human): S397‑p, YAP1 iso2 (human): S381‑p, YAP1 iso3 (human): S347‑p, YAP1 (mouse): S382‑p, YAP1 (rat): S363‑p, YAP1 iso3 (rat): , YAP1 (sheep): S296‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LATS1 (human) transfection of wild-type enzyme, mutation in upstream enzyme recognition motif
Downstream Regulation
 Effect of modification (function):  phosphorylation, protein degradation, ubiquitination
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BTRC (human) Induces co-immunoprecipitation
 Comments:  S381 phosphorylation primes for S384 phosphorylation

S384-p - YAP1 iso2 (human)
Orthologous residues
YAP1 (human): S400‑p, YAP1 iso2 (human): S384‑p, YAP1 iso3 (human): S350‑p, YAP1 (mouse): S385‑p, YAP1 (rat): S366‑p, YAP1 iso3 (rat): , YAP1 (sheep): S299‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK1D (human) transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme, transfection of inactive enzyme
Downstream Regulation
 Effect of modification (function):  ubiquitination
 Effect of modification (process):  carcinogenesis, inhibited
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BTRC (human) Induces co-immunoprecipitation

S387-p - YAP1 iso2 (human)
Orthologous residues
YAP1 (human): S403‑p, YAP1 iso2 (human): S387‑p, YAP1 iso3 (human): S353‑p, YAP1 (mouse): S388‑p, YAP1 (rat): S369‑p, YAP1 iso3 (rat): , YAP1 (sheep): S302‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  ubiquitination
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BTRC (human) Induces co-immunoprecipitation


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