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Curated Information Page
PubMed Id: 20064930 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Huang YZ, McNamara JO (2010) Mutual regulation of Src family kinases and the neurotrophin receptor TrkB. J Biol Chem 285, 8207-17 20064930
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

Y516-p - TrkB (human) ▲
Orthologous residues
TrkB (human): Y516‑p, TrkB iso4 (human): Y532‑p, TrkB (mouse): Y515‑p, TrkB iso2 (mouse): ‑, TrkB (rat): Y515‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebral cortical'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE TrkB (human)

T203-p - ERK1 (mouse) ▲
Orthologous residues
ERK1 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebral cortical'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
1Na-PP1 BDNF inhibit treatment-induced increase
Zn(2+) increase
1Na-PP1 Zn(2+) no effect upon treatment-induced increase
BDNF increase
SMI11293 BDNF no effect upon treatment-induced increase

Y205-p - ERK1 (mouse) ▲
Orthologous residues
ERK1 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebral cortical'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
1Na-PP1 BDNF inhibit treatment-induced increase
Zn(2+) increase
1Na-PP1 Zn(2+) no effect upon treatment-induced increase
BDNF increase
SMI11293 BDNF no effect upon treatment-induced increase

T183-p - ERK2 (mouse) ▲
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebral cortical'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
1Na-PP1 BDNF inhibit treatment-induced increase
Zn(2+) increase
1Na-PP1 Zn(2+) no effect upon treatment-induced increase
BDNF increase
SMI11293 BDNF no effect upon treatment-induced increase

Y185-p - ERK2 (mouse) ▲
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebral cortical'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
1Na-PP1 BDNF inhibit treatment-induced increase
Zn(2+) increase
1Na-PP1 Zn(2+) no effect upon treatment-induced increase
BDNF increase
SMI11293 BDNF no effect upon treatment-induced increase

Y424-p - Src (mouse) ▲
Orthologous residues
Src (human): Y419‑p, Src (mouse): Y424‑p, Src iso2 (mouse): Y418‑p, Src (rat): Y419‑p, Src (chicken): Y416‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebral cortical'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
1Na-PP1 BDNF inhibit treatment-induced increase
PP1 BDNF inhibit treatment-induced increase
Zn(2+) increase
1Na-PP1 Zn(2+) inhibit treatment-induced increase slight inhibition
PP1 Zn(2+) inhibit treatment-induced increase

Y515-p - TrkB (mouse) ▲
Orthologous residues
TrkB (human): Y516‑p, TrkB iso4 (human): Y532‑p, TrkB (mouse): Y515‑p, TrkB iso2 (mouse): ‑, TrkB (rat): Y515‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebral cortical'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
1Na-PP1 BDNF inhibit treatment-induced increase
Zn(2+) increase
1Na-PP1 Zn(2+) no effect upon treatment-induced increase
BDNF increase
1Na-PP1 BDNF inhibit treatment-induced increase
PP1 BDNF inhibit treatment-induced increase
Zn(2+) increase
1Na-PP1 Zn(2+) inhibit treatment-induced increase slight inhibition
PP1 Zn(2+) inhibit treatment-induced increase

Y705-p - TrkB (mouse) ▲
Orthologous residues
TrkB (human): Y706‑p, TrkB iso4 (human): Y722‑p, TrkB (mouse): Y705‑p, TrkB iso2 (mouse): ‑, TrkB (rat): Y705‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebral cortical'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
1Na-PP1 BDNF inhibit treatment-induced increase
Zn(2+) increase
1Na-PP1 Zn(2+) no effect upon treatment-induced increase
BDNF increase
1Na-PP1 BDNF inhibit treatment-induced increase
PP1 BDNF inhibit treatment-induced increase
Zn(2+) increase
1Na-PP1 Zn(2+) inhibit treatment-induced increase slight inhibition
PP1 Zn(2+) inhibit treatment-induced increase

Y706-p - TrkB (mouse) ▲
Orthologous residues
TrkB (human): Y707‑p, TrkB iso4 (human): Y723‑p, TrkB (mouse): Y706‑p, TrkB iso2 (mouse): ‑, TrkB (rat): Y706‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebral cortical'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
1Na-PP1 BDNF inhibit treatment-induced increase
Zn(2+) increase
1Na-PP1 Zn(2+) no effect upon treatment-induced increase
BDNF increase
1Na-PP1 BDNF inhibit treatment-induced increase
PP1 BDNF inhibit treatment-induced increase
Zn(2+) increase
1Na-PP1 Zn(2+) inhibit treatment-induced increase slight inhibition
PP1 Zn(2+) inhibit treatment-induced increase

S473-p - Akt1 (rat) ▲
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebral cortical'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
SMI11293 BDNF no effect upon treatment-induced increase

Y771-p - PLCG1 (rat) ▲
Orthologous residues
PLCG1 (human): Y771‑p, PLCG1 iso2 (human): Y771‑p, PLCG1 (mouse): Y771‑p, PLCG1 (rat): Y771‑p, PLCG1 (cow): Y771‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebral cortical'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
SMI11293 BDNF inhibit treatment-induced increase

Y783-p - PLCG1 (rat) ▲
Orthologous residues
PLCG1 (human): Y783‑p, PLCG1 iso2 (human): Y783‑p, PLCG1 (mouse): Y783‑p, PLCG1 (rat): Y783‑p, PLCG1 (cow): Y783‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebral cortical'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
SMI11293 BDNF inhibit treatment-induced increase

Y419-p - Src (rat) ▲
Orthologous residues
Src (human): Y419‑p, Src (mouse): Y424‑p, Src iso2 (mouse): Y418‑p, Src (rat): Y419‑p, Src (chicken): Y416‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebral cortical'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Src (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
SMI11293 BDNF inhibit treatment-induced increase
CGP76030 BDNF inhibit treatment-induced increase
Zn(2+) increase
SMI11293 Zn(2+) inhibit treatment-induced increase
CGP76030 Zn(2+) inhibit treatment-induced increase
Zn(2+) increase
PP1 Zn(2+) inhibit treatment-induced increase
PP2 Zn(2+) inhibit treatment-induced increase
PP3 Zn(2+) no effect upon treatment-induced increase
TrkB (mouse) increase TrkB knockout decreases

Y530-p - Src (rat) ▲
Orthologous residues
Src (human): Y530‑p, Src (mouse): Y535‑p, Src iso2 (mouse): Y529‑p, Src (rat): Y530‑p, Src (chicken): Y527‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebral cortical'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
PP1 BDNF inhibit treatment-induced increase
Zn(2+) decrease

Y515-p - TrkB (rat) ▲
Orthologous residues
TrkB (human): Y516‑p, TrkB iso4 (human): Y532‑p, TrkB (mouse): Y515‑p, TrkB iso2 (mouse): ‑, TrkB (rat): Y515‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebral cortical'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE TrkB (rat)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TrkB (rat) pharmacological activator of upstream enzyme, mutation in upstream enzyme recognition motif
 Comments:  autophosphorylation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
SMI11293 BDNF inhibit treatment-induced increase
CGP76030 BDNF inhibit treatment-induced increase
Zn(2+) increase
SMI11293 Zn(2+) inhibit treatment-induced increase
CGP76030 Zn(2+) inhibit treatment-induced increase
BDNF increase
K252a BDNF inhibit treatment-induced increase
Zn(2+) increase
K252a Zn(2+) inhibit treatment-induced increase
K252a no change compared to control
Src (rat) increase inactive Src decreases

Y705-p - TrkB (rat) ▲
Orthologous residues
TrkB (human): Y706‑p, TrkB iso4 (human): Y722‑p, TrkB (mouse): Y705‑p, TrkB iso2 (mouse): ‑, TrkB (rat): Y705‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebral cortical'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Src (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TrkB (rat) pharmacological activator of upstream enzyme, mutation in upstream enzyme recognition motif
 Comments:  autophosphorylation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
SMI11293 BDNF inhibit treatment-induced increase
CGP76030 BDNF inhibit treatment-induced increase
Zn(2+) increase
SMI11293 Zn(2+) inhibit treatment-induced increase
CGP76030 Zn(2+) inhibit treatment-induced increase
BDNF increase
K252a BDNF inhibit treatment-induced increase slight inhibition
Zn(2+) increase
K252a Zn(2+) no effect upon treatment-induced increase
K252a no change compared to control
Src (rat) increase inactive Src decreases
Zn(2+) increase
PP1 Zn(2+) inhibit treatment-induced increase
PP2 Zn(2+) inhibit treatment-induced increase
PP3 Zn(2+) no effect upon treatment-induced increase

Y706-p - TrkB (rat) ▲
Orthologous residues
TrkB (human): Y707‑p, TrkB iso4 (human): Y723‑p, TrkB (mouse): Y706‑p, TrkB iso2 (mouse): ‑, TrkB (rat): Y706‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cerebral cortical'-brain
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Src (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TrkB (rat) pharmacological activator of upstream enzyme, mutation in upstream enzyme recognition motif
 Comments:  autophosphorylation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
SMI11293 BDNF inhibit treatment-induced increase
CGP76030 BDNF inhibit treatment-induced increase
Zn(2+) increase
SMI11293 Zn(2+) inhibit treatment-induced increase
CGP76030 Zn(2+) inhibit treatment-induced increase
BDNF increase
K252a BDNF inhibit treatment-induced increase slight inhibition
Zn(2+) increase
K252a Zn(2+) no effect upon treatment-induced increase
K252a no change compared to control
Src (rat) increase inactive Src decreases
Zn(2+) increase
PP1 Zn(2+) inhibit treatment-induced increase
PP2 Zn(2+) inhibit treatment-induced increase
PP3 Zn(2+) no effect upon treatment-induced increase


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