Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Curated Information Page
PubMed Id: 11583630 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Lee MJ, et al. (2001) Akt-mediated phosphorylation of the G protein-coupled receptor EDG-1 is required for endothelial cell chemotaxis. Mol Cell 8, 693-704 11583630
Download Sites

T236-p - EDG-1 (human)
Orthologous residues
EDG‑1 (human): T236‑p, EDG‑1 (mouse): T236‑p, EDG‑1 (rat): T237‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  CHO (fibroblast) [EphB1 (human), transfection], HUVEC (endothelial), muscle
 Cellular systems studied:  cell lines, primary cells
 Species studied:  hamster, human, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) pharmacological activator of upstream enzyme, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sphingosin 1-phosphate Akt1 (human) increase
LY294002 sphingosin 1-phosphate Akt1 (human) inhibit treatment-induced increase
wortmannin sphingosin 1-phosphate Akt1 (human) inhibit treatment-induced increase
IGF-1 increase
Downstream Regulation
 Effect of modification (process):  cell motility, altered, cytoskeletal reorganization
 Comments:  Cortical actin assembly, chemotaxis, and angiogenesis


Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.