Curated Information
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Curated Information Page
PubMed Id: 11058583 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Daub H, et al. (2001) Rac/Cdc42 and p65PAK regulate the microtubule-destabilizing protein stathmin through phosphorylation at serine 16. J Biol Chem 276, 1677-80 11058583
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S16-p - STMN1 (human) ▲
Orthologous residues
STMN1 (human): S16‑p, STMN1 (mouse): S16‑p, STMN1 (rat): S16‑p, STMN1 (chicken): S16‑p
Characterization
 Methods used to characterize site in vivo:  2D analysis, phospho-antibody
 Relevant cell lines - cell types - tissues:  HEp-2 (squamous)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PAK1 (human) inhibition of upstream enzyme Inhibited PAK65 by transfection of autoinhibitory fragment (residues 83-149) of p65PAK. Whether p65PAK directly phosphorylates stathmin or whether it activates another downstream kinase is not currently known.
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S25-p - STMN1 (human) ▲
Orthologous residues
STMN1 (human): S25‑p, STMN1 (mouse): S25‑p, STMN1 (rat): S25‑p, STMN1 (chicken): G25‑p
Characterization
 Methods used to characterize site in vivo:  2D analysis, phospho-antibody
 Relevant cell lines - cell types - tissues:  HEp-2 (squamous)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S38-p - STMN1 (human) ▲
Orthologous residues
STMN1 (human): S38‑p, STMN1 (mouse): S38‑p, STMN1 (rat): S38‑p, STMN1 (chicken): S38‑p
Characterization
 Methods used to characterize site in vivo:  2D analysis, phospho-antibody
 Relevant cell lines - cell types - tissues:  HEp-2 (squamous)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF no change compared to control S37 is constitutively phosphorylated in HEp-2 cells.

S63-p - STMN1 (human) ▲
Orthologous residues
STMN1 (human): S63‑p, STMN1 (mouse): S63‑p, STMN1 (rat): S63‑p, STMN1 (chicken): S63‑p
Characterization
 Methods used to characterize site in vivo:  2D analysis
 Relevant cell lines - cell types - tissues:  HEp-2 (squamous)
 Cellular systems studied:  cell lines
 Species studied:  human


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