Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Curated Information Page
PubMed Id: 20178744 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Woo HA, et al. (2010) Inactivation of peroxiredoxin I by phosphorylation allows localized H(2)O(2) accumulation for cell signaling. Cell 140, 517-28 20178744
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

Y194-p - NKEF-A (human) ▲
Orthologous residues
NKEF‑A (human): Y194‑p, NKEF‑A (mouse): Y194‑p, NKEF‑A (rat): Y194‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  lymphoma, Burkitt's lymphoma
 Relevant cell lines - cell types - tissues:  A431 (epithelial), Jurkat (T lymphocyte), RAMOS (B lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Lck (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
AG1478 EGF inhibit treatment-induced increase
PP1 EGF inhibit treatment-induced increase
anti-IgM increase
PP1 anti-IgM inhibit treatment-induced increase
anti-CD3 increase
PDGF NOXO1 (human), NOXA1 (human), NOX1 (human) increase decreased in NOX1/NOXA1/NOXO1 knockout
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited, intracellular localization, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
NOX1 (human) Induces electrophoretic visualization

Y194-p - NKEF-A (mouse) ▲
Orthologous residues
NKEF‑A (human): Y194‑p, NKEF‑A (mouse): Y194‑p, NKEF‑A (rat): Y194‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast), 3T3 (fibroblast) [EGFR (human), transfection], MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (mouse) siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
PDGF NOX1 (mouse) increase decreased in NOX1 knockout mice
PDGF Src (mouse) increase c-Src siRNA decreases
STI571 PDGF inhibit treatment-induced increase
EGF NOX1 (mouse) increase decreased in NOX1 knockout mice
wounding increase

Y194-p - NKEF-A (rat) ▲
Orthologous residues
NKEF‑A (human): Y194‑p, NKEF‑A (mouse): Y194‑p, NKEF‑A (rat): Y194‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, smooth'
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase


Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.