Curated Information
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Curated Information Page
PubMed Id: 20169205 
Gwinn DM, Asara JM, Shaw RJ (2010) Raptor is phosphorylated by cdc2 during mitosis. PLoS One 5, e9197 20169205
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S696-p - Raptor (human)
Orthologous residues
Raptor (human): S696‑p, Raptor (mouse): S696‑p, Raptor (rat): S696‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  lung cancer
 Relevant cell lines - cell types - tissues:  293T (epithelial), A549 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  in mitotic cells
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
taxol increase

T706-p - Raptor (human)
Orthologous residues
Raptor (human): T706‑p, Raptor (mouse): T706‑p, Raptor (rat): T706‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  lung cancer
 Relevant cell lines - cell types - tissues:  293T (epithelial), A549 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  in mitotic cells
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK1 (human) co-immunoprecipitation, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
roscovitine nocodazole inhibit treatment-induced increase

S711-p - Raptor (human)
Orthologous residues
Raptor (human): S711‑p, Raptor (mouse): S711‑p, Raptor (rat): S711‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S719-p - Raptor (human)
Orthologous residues
Raptor (human): S719‑p, Raptor (mouse): S719‑p, Raptor (rat): S719‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S721-p - Raptor (human)
Orthologous residues
Raptor (human): S721‑p, Raptor (mouse): S721‑p, Raptor (rat): S721‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S722-p - Raptor (human)
Orthologous residues
Raptor (human): S722‑p, Raptor (mouse): S722‑p, Raptor (rat): S722‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

Y723-p - Raptor (human)
Orthologous residues
Raptor (human): Y723‑p, Raptor (mouse): Y723‑p, Raptor (rat): Y723‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S738-p - Raptor (human)
Orthologous residues
Raptor (human): S738‑p, Raptor (mouse): S738‑p, Raptor (rat): S738‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S771-p - Raptor (human)
Orthologous residues
Raptor (human): S771‑p, Raptor (mouse): S771‑p, Raptor (rat): S771‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S792-p - Raptor (human)
Orthologous residues
Raptor (human): S792‑p, Raptor (mouse): S792‑p, Raptor (rat): S792‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S795-p - Raptor (human)
Orthologous residues
Raptor (human): S795‑p, Raptor (mouse): A795‑p, Raptor (rat): A795‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

T853-p - Raptor (human)
Orthologous residues
Raptor (human): T853‑p, Raptor (mouse): T853‑p, Raptor (rat): T853‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S855-p - Raptor (human)
Orthologous residues
Raptor (human): S855‑p, Raptor (mouse): S855‑p, Raptor (rat): S855‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

T857-p - Raptor (human)
Orthologous residues
Raptor (human): T857‑p, Raptor (mouse): T857‑p, Raptor (rat): T857‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S859-p - Raptor (human)
Orthologous residues
Raptor (human): S859‑p, Raptor (mouse): S859‑p, Raptor (rat): S859‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S863-p - Raptor (human)
Orthologous residues
Raptor (human): S863‑p, Raptor (mouse): S863‑p, Raptor (rat): S863‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

T865-p - Raptor (human)
Orthologous residues
Raptor (human): T865‑p, Raptor (mouse): T865‑p, Raptor (rat): T865‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S877-p - Raptor (human)
Orthologous residues
Raptor (human): S877‑p, Raptor (mouse): S877‑p, Raptor (rat): S877‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S881-p - Raptor (human)
Orthologous residues
Raptor (human): S881‑p, Raptor (mouse): S881‑p, Raptor (rat): S881‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

T883-p - Raptor (human)
Orthologous residues
Raptor (human): T883‑p, Raptor (mouse): T883‑p, Raptor (rat): T883‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S886-p - Raptor (human)
Orthologous residues
Raptor (human): S886‑p, Raptor (mouse): C886‑p, Raptor (rat): C886‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

T889-p - Raptor (human)
Orthologous residues
Raptor (human): T889‑p, Raptor (mouse): T889‑p, Raptor (rat): T889‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human


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