Curated Information
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Curated Information Page
PubMed Id: 20142099 
Yamada E, et al. (2010) Fyn-dependent regulation of energy expenditure and body weight is mediated by tyrosine phosphorylation of LKB1. Cell Metab 11, 113-24 20142099
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S79-p - ACC1 (mouse)
Orthologous residues
ACC1 (human): S80‑p, ACC1 iso4 (human): S117‑p, ACC1 (mouse): S79‑p, ACC1 iso2 (mouse): S117‑p, ACC1 (rat): S79‑p, ACC1 iso2 (rat): S79‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  muscle
 Cellular systems studied:  tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SU6656 increase

S212-p - ACC2 (mouse)
Orthologous residues
ACC2 (human): S222‑p, ACC2 (mouse): S212‑p, ACC2 (rat): S218‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  muscle
 Cellular systems studied:  tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SU6656 increase

T172-p - AMPKA2 (mouse)
Orthologous residues
AMPKA2 (human): T172‑p, AMPKA2 (mouse): T172‑p, AMPKA2 (rat): T172‑p, AMPKA2 (pig): T172‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  muscle
 Cellular systems studied:  tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SU6656 increase
LKB1 (mouse) increase LKB1 Y261/365F double mutant

Y60-p - LKB1 (mouse)
Orthologous residues
LKB1 (human): Y60‑p, LKB1 iso2 (human): Y60‑p, LKB1 (mouse): Y60‑p, LKB1 (rat): Y60‑p, LKB1 (pig):
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Fyn (human)

Y156-p - LKB1 (mouse)
Orthologous residues
LKB1 (human): Y156‑p, LKB1 iso2 (human): Y156‑p, LKB1 (mouse): Y156‑p, LKB1 (rat): Y156‑p, LKB1 (pig): Y62‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Fyn (human)

Y166-p - LKB1 (mouse)
Orthologous residues
LKB1 (human): Y166‑p, LKB1 iso2 (human): Y166‑p, LKB1 (mouse): Y166‑p, LKB1 (rat): Y166‑p, LKB1 (pig): Y72‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Fyn (human)

Y261-p - LKB1 (mouse)
Orthologous residues
LKB1 (human): Y261‑p, LKB1 iso2 (human): Y261‑p, LKB1 (mouse): Y261‑p, LKB1 (rat): Y261‑p, LKB1 (pig): Y167‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), C2C12 (myoblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Fyn (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Fyn (human) transfection of inactive enzyme, transfection of constitutively active enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SU6656 decrease
Downstream Regulation
 Effect of modification (function):  intracellular localization, phosphorylation
 Comments:  mutation of this site increses AMPK phosphorylation

Y365-p - LKB1 (mouse)
Orthologous residues
LKB1 (human): Y362‑p, LKB1 iso2 (human): Y362‑p, LKB1 (mouse): Y365‑p, LKB1 (rat): Y365‑p, LKB1 (pig): Y270‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), C2C12 (myoblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Fyn (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Fyn (human) transfection of inactive enzyme, transfection of constitutively active enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SU6656 decrease
Downstream Regulation
 Effect of modification (function):  intracellular localization, phosphorylation
 Comments:  mutation of this site increses AMPK phosphorylation


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