Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Curated Information Page
PubMed Id: 19920251 
Hitosugi T, et al. (2009) Tyrosine phosphorylation inhibits PKM2 to promote the Warburg effect and tumor growth. Sci Signal 2, ra73 19920251
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

Y83-p - PKM2 (mouse)
Orthologous residues
PKM2 (human): Y83‑p, PKM2 iso2 (human): Y83‑p, PKM2 iso3 (human): Y68‑p, PKM2 (mouse): Y83‑p, PKM2 (rat): Y83‑p, PKM2 iso2 (rat): Y83‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  293T (epithelial) [FGFR1 (human), transfection], BaF3 ('B lymphocyte, precursor') [FGFR1 (human), transfection], NCI-H1299 (pulmonary) [FGFR1 (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Comments:  constitutively active ZNF198-FGFR1 transfected cells

Y105-p - PKM2 (mouse)
Orthologous residues
PKM2 (human): Y105‑p, PKM2 iso2 (human): Y105‑p, PKM2 iso3 (human): Y90‑p, PKM2 (mouse): Y105‑p, PKM2 (rat): Y105‑p, PKM2 iso2 (rat): Y105‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  breast cancer, leukemia, acute myelogenous leukemia, chronic myelogenous leukemia, erythroid leukemia, lung cancer, non-small cell lung cancer, prostate cancer
 Relevant cell lines - cell types - tissues:  293T (epithelial) [FGFR1 (human), transfection], A549 (pulmonary), BaF3 ('B lymphocyte, precursor') [FGFR1 (human), transfection], DU 145 (prostate cell), HEL (erythroid), K562 (erythroid), KG-1a (myeloid), MDA-MB231 (breast cell), MO-91 (myeloid), Molm 14 (myeloid), NCI-H1299 (pulmonary) [FGFR1 (human), transfection], PC3 (prostate cell)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Comments:  constitutively active ZNF198-FGFR1 transfected cells
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE FGFR1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE FGFR1 (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
imatinib decrease
AG490 decrease
TKI258 decrease
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited, molecular association, regulation
 Effect of modification (process):  carcinogenesis, induced, cell growth, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PKM2 (human) Disrupts chemical cross-linking
 Comments:  disrupts tetramerization of PKM2

Y148-p - PKM2 (mouse)
Orthologous residues
PKM2 (human): Y148‑p, PKM2 iso2 (human): Y148‑p, PKM2 iso3 (human): Y133‑p, PKM2 (mouse): Y148‑p, PKM2 (rat): Y148‑p, PKM2 iso2 (rat): Y148‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  293T (epithelial) [FGFR1 (human), transfection], BaF3 ('B lymphocyte, precursor') [FGFR1 (human), transfection], NCI-H1299 (pulmonary) [FGFR1 (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Comments:  constitutively active ZNF198-FGFR1 transfected cells

Y175-p - PKM2 (mouse)
Orthologous residues
PKM2 (human): Y175‑p, PKM2 iso2 (human): Y175‑p, PKM2 iso3 (human): Y160‑p, PKM2 (mouse): Y175‑p, PKM2 (rat): Y175‑p, PKM2 iso2 (rat): Y175‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  293T (epithelial) [FGFR1 (human), transfection], BaF3 ('B lymphocyte, precursor') [FGFR1 (human), transfection], NCI-H1299 (pulmonary) [FGFR1 (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Comments:  constitutively active ZNF198-FGFR1 transfected cells

Y370-p - PKM2 (mouse)
Orthologous residues
PKM2 (human): Y370‑p, PKM2 iso2 (human): Y370‑p, PKM2 iso3 (human): Y355‑p, PKM2 (mouse): Y370‑p, PKM2 (rat): Y370‑p, PKM2 iso2 (rat): Y370‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  293T (epithelial) [FGFR1 (human), transfection], BaF3 ('B lymphocyte, precursor') [FGFR1 (human), transfection], NCI-H1299 (pulmonary) [FGFR1 (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Comments:  constitutively active ZNF198-FGFR1 transfected cells

Y390-p - PKM2 (mouse)
Orthologous residues
PKM2 (human): Y390‑p, PKM2 iso2 (human): , PKM2 iso3 (human): Y375‑p, PKM2 (mouse): Y390‑p, PKM2 (rat): , PKM2 iso2 (rat): Y390‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  293T (epithelial) [FGFR1 (human), transfection], BaF3 ('B lymphocyte, precursor') [FGFR1 (human), transfection], NCI-H1299 (pulmonary) [FGFR1 (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Comments:  constitutively active ZNF198-FGFR1 transfected cells


Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.