Curated Information
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PubMed Id: 9380693 
Liu X, et al. (1997) Transforming growth factor beta-induced phosphorylation of Smad3 is required for growth inhibition and transcriptional induction in epithelial cells. Proc Natl Acad Sci U S A 94, 10669-74 9380693
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T8-p - Smad3 (human)
Orthologous residues
Smad3 (human): T8‑p, Smad3 (mouse): T8‑p, Smad3 (rat): T8‑p

T179-p - Smad3 (human)
Orthologous residues
Smad3 (human): T179‑p, Smad3 (mouse): T179‑p, Smad3 (rat): T179‑p

S204-p - Smad3 (human)
Orthologous residues
Smad3 (human): S204‑p, Smad3 (mouse): S204‑p, Smad3 (rat): S204‑p

S208-p - Smad3 (human)
Orthologous residues
Smad3 (human): S208‑p, Smad3 (mouse): S208‑p, Smad3 (rat): S208‑p

S213-p - Smad3 (human)
Orthologous residues
Smad3 (human): S213‑p, Smad3 (mouse): S213‑p, Smad3 (rat): S213‑p

S422-p - Smad3 (human)
Orthologous residues
Smad3 (human): S422‑p, Smad3 (mouse): S422‑p, Smad3 (rat): S422‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  Mv1Lu (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  mink
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TGF-beta increase
Downstream Regulation
 Effect of modification (process):  transcription, altered
 Comments:  The triple mutants(S422/423/425A and S422/423/425D) were used in these experiments

S423-p - Smad3 (human)
Orthologous residues
Smad3 (human): S423‑p, Smad3 (mouse): S423‑p, Smad3 (rat): S423‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  Mv1Lu (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  mink
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TGF-beta increase
Downstream Regulation
 Effect of modification (process):  transcription, altered
 Comments:  The triple mutants(S422/423/425A and S422/423/425D) were used in these experiments

S425-p - Smad3 (human)
Orthologous residues
Smad3 (human): S425‑p, Smad3 (mouse): S425‑p, Smad3 (rat): S425‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  Mv1Lu (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  mink
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TGF-beta increase
Downstream Regulation
 Effect of modification (process):  transcription, altered
 Comments:  The triple mutants(S422/423/425A and S422/423/425D) were used in these experiments


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