Curated Information
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Curated Information Page
PubMed Id: 8939605 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
van der Geer P, Wiley S, Gish GD, Pawson T (1996) The Shc adaptor protein is highly phosphorylated at conserved, twin tyrosine residues (Y239/240) that mediate protein-protein interactions. Curr Biol 6, 1435-44 8939605
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y349-p - Shc1 (human) ▲
Orthologous residues
Shc1 (human): Y349‑p, Shc1 iso2 (human): Y239‑p, Shc1 iso3 (human): Y194‑p, Shc1 iso6 (human): Y349‑p, Shc1 iso7 (human): Y239‑p, Shc1 (mouse): Y349‑p, Shc1 iso2 (mouse): Y239‑p, Shc1 iso3 (mouse): Y194‑p, Shc1 (rat): Y349‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Src (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Grb2 (human) SH2 Induces co-immunoprecipitation

Y350-p - Shc1 (human) ▲
Orthologous residues
Shc1 (human): Y350‑p, Shc1 iso2 (human): Y240‑p, Shc1 iso3 (human): Y195‑p, Shc1 iso6 (human): Y350‑p, Shc1 iso7 (human): Y240‑p, Shc1 (mouse): Y350‑p, Shc1 iso2 (mouse): Y240‑p, Shc1 iso3 (mouse): Y195‑p, Shc1 (rat): Y350‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Src (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Grb2 (human) SH2 Induces co-immunoprecipitation

Y427-p - Shc1 (human) ▲
Orthologous residues
Shc1 (human): Y427‑p, Shc1 iso2 (human): Y317‑p, Shc1 iso3 (human): Y272‑p, Shc1 iso6 (human): Y428‑p, Shc1 iso7 (human): Y318‑p, Shc1 (mouse): Y423‑p, Shc1 iso2 (mouse): Y313‑p, Shc1 iso3 (mouse): Y268‑p, Shc1 (rat): Y423‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase


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