Curated Information
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Curated Information Page
PubMed Id: 10806207 
Tomás-Zuber M, Mary JL, Lesslauer W (2000) Control sites of ribosomal S6 kinase B and persistent activation through tumor necrosis factor. J Biol Chem 275, 23549-58 10806207
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S196-p - MSK2 (human)
Orthologous residues
MSK2 (human): S196‑p, MSK2 iso2 (human): S196‑p, MSK2 (mouse): S196‑p, MSK2 (rat): S196‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, peptide sequencing
 Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

S343-p - MSK2 (human)
Orthologous residues
MSK2 (human): S343‑p, MSK2 iso2 (human): S343‑p, MSK2 (mouse): S343‑p, MSK2 (rat): S343‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, peptide sequencing
 Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

S347-p - MSK2 (human)
Orthologous residues
MSK2 (human): S347‑p, MSK2 iso2 (human): S347‑p, MSK2 (mouse): S347‑p, MSK2 (rat): S347‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, peptide sequencing, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
P38A (human), MKK6 (mouse) increase
SB202190 MKK6 (mouse), P38A (human) inhibit treatment-induced increase
TNF increase
SB202190 TNF inhibit treatment-induced increase
PD98059 TNF no effect upon treatment-induced increase
ionomycin increase
SB202190 ionomycin inhibit treatment-induced increase
PD98059 ionomycin no effect upon treatment-induced increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

S360-p - MSK2 (human)
Orthologous residues
MSK2 (human): S360‑p, MSK2 iso2 (human): S360‑p, MSK2 (mouse): S360‑p, MSK2 (rat): S360‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, peptide sequencing, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
P38A (human), MKK6 (mouse) increase
SB202190 MKK6 (mouse), P38A (human) inhibit treatment-induced increase
TNF increase
SB202190 TNF inhibit treatment-induced increase
PD98059 TNF no effect upon treatment-induced increase
ionomycin increase
SB202190 ionomycin inhibit treatment-induced increase
PD98059 ionomycin no effect upon treatment-induced increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

T568-p - MSK2 (human)
Orthologous residues
MSK2 (human): T568‑p, MSK2 iso2 (human): T562‑p, MSK2 (mouse): T568‑p, MSK2 (rat): T568‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, peptide sequencing
 Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced


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