Curated Information
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PubMed Id: 20086174 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Romano D, et al. (2010) Proapoptotic kinase MST2 coordinates signaling crosstalk between RASSF1A, Raf-1, and Akt. Cancer Res 70, 1195-203 20086174
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S73-p - Jun (human)
Orthologous residues
Jun (human): S73‑p, Jun (mouse): S73‑p, Jun (rat): S73‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  HCC1937 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MST2 (human) decrease phosphorylation of this site increases in the double MST2 T117/384A mutant
MST2 (human) increase phosphorylation of this site decreases in the MST2 T180A mutant

T117-p - MST2 (human)
Orthologous residues
MST2 (human): T117‑p, MST2 (mouse): T117‑p, MST2 (rat): T117‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  HCC1937 (breast cell), HeLa (cervical), MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) modification site within consensus motif, phospho-motif antibody
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LY294002 decrease
Akt-I-1 decrease
serum increase
LY294002 serum inhibit treatment-induced increase
IGF-1 increase
LY294002 IGF-1 inhibit treatment-induced increase
PTEN (human) decrease
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited, molecular association, regulation
 Effect of modification (process):  apoptosis, altered, signaling pathway regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
RAF1 (human) Induces co-immunoprecipitation
MST2 (human) Disrupts co-immunoprecipitation
RASSF1 (human) Disrupts co-immunoprecipitation

T180-p - MST2 (human)
Orthologous residues
MST2 (human): T180‑p, MST2 (mouse): T180‑p, MST2 (rat): T180‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  HCC1937 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Effect of modification (process):  signaling pathway regulation

T384-p - MST2 (human)
Orthologous residues
MST2 (human): T384‑p, MST2 (mouse): T390‑p, MST2 (rat): T384‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  HCC1937 (breast cell), HeLa (cervical), MCF-7 (breast cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) modification site within consensus motif, phospho-motif antibody
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LY294002 decrease
Akt-I-1 decrease
serum increase
LY294002 serum inhibit treatment-induced increase
IGF-1 increase
LY294002 IGF-1 inhibit treatment-induced increase
PTEN (human) decrease
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited, molecular association, regulation
 Effect of modification (process):  apoptosis, altered, signaling pathway regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
RASSF1 (human) Disrupts co-immunoprecipitation
MST2 (human) Disrupts co-immunoprecipitation
RAF1 (human) Induces co-immunoprecipitation


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