|
Orthologous residues
|
|
Kv4.2 (human): S552‑p, Kv4.2 (mouse): S552‑p, Kv4.2 (rat): S552‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
immunoprecipitation, mutation of modification site, western blotting
|
|
Relevant cell lines - cell types - tissues:
293 (epithelial) [KCNIP4 (mouse), transfection], COS (fibroblast) [KCNIP4 (mouse), transfection]
|
|
Cellular systems studied:
cell lines
|
|
Species studied:
human, monkey
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
PKACa (human)
|
pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme
|
|
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
forskolin
|
|
|
|
increase
|
|
|
H-89
|
forskolin
|
|
|
inhibit treatment-induced increase
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
intracellular localization, protein stabilization
|
|
Comments:
KCNIP4-mediated enhancement of channel surface expression
|
