Curated Information
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Curated Information Page
PubMed Id: 20068038 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Sun W, et al. (2010) Phosphorylation of Thr-516 and Ser-520 in the kinase activation loop of MEKK3 is required for lysophosphatidic acid-mediated optimal IkappaB kinase beta (IKKbeta)/nuclear factor-kappaB (NF-kappaB) activation. J Biol Chem 285, 7911-8 20068038
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S177-p - IKK-beta (human) ▲
Orthologous residues
IKK‑beta (human): S177‑p, IKK‑beta (mouse): S177‑p, IKK‑beta (rat): S177‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MEKK3 (human) increase

S181-p - IKK-beta (human) ▲
Orthologous residues
IKK‑beta (human): S181‑p, IKK‑beta (mouse): S181‑p, IKK‑beta (rat): S181‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MEKK3 (human) increase

T516-p - MEKK3 (human) ▲
Orthologous residues
MEKK3 (human): T516‑p, MEKK3 (mouse): T516‑p, MEKK3 (rat): T516‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPA increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Comments:  essential for MEKK3-mediated IKK-beta/NFkB activation

S520-p - MEKK3 (human) ▲
Orthologous residues
MEKK3 (human): S520‑p, MEKK3 (mouse): S520‑p, MEKK3 (rat): S520‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPA increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Comments:  essential for MEKK3-mediated IKK-beta/NFkB activation

S526-p - MEKK3 (human) ▲
Orthologous residues
MEKK3 (human): S526‑p, MEKK3 (mouse): S526‑p, MEKK3 (rat): S526‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse


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