Curated Information
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Curated Information Page
PubMed Id: 20051463 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Larance M, et al. (2010) Global phosphoproteomics identifies a major role for AKT and 14-3-3 in regulating EDC3. Mol Cell Proteomics 9, 682-94 20051463
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S109-p - EDC3 (human) ▲
Orthologous residues
EDC3 (human): S109‑p, EDC3 (mouse): S109‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry
 Relevant cell lines - cell types - tissues:  CHO (fibroblast) [InsR (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  hamster
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

S131-p - EDC3 (human) ▲
Orthologous residues
EDC3 (human): S131‑p, EDC3 (mouse): S131‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry
 Relevant cell lines - cell types - tissues:  CHO (fibroblast) [InsR (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  hamster
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

S161-p - EDC3 (human) ▲
Orthologous residues
EDC3 (human): S161‑p, EDC3 (mouse): S161‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast), CHO (fibroblast) [InsR (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  hamster, mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Akt2 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) pharmacological inhibitor of upstream enzyme, modification site within consensus motif, phospho-motif antibody, pharmacological activator of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
wortmannin insulin inhibit treatment-induced increase
Akt-I-1 insulin inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces co-immunoprecipitation

T173-p - EDC3 (human) ▲
Orthologous residues
EDC3 (human): T173‑p, EDC3 (mouse): T173‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry
 Relevant cell lines - cell types - tissues:  CHO (fibroblast) [InsR (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  hamster
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

Y225-p - EDC3 (human) ▲
Orthologous residues
EDC3 (human): Y225‑p, EDC3 (mouse): Y225‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry
 Relevant cell lines - cell types - tissues:  CHO (fibroblast) [InsR (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  hamster
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase


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