Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Curated Information Page
PubMed Id: 20048153 
Busillo JM, et al. (2010) Site-specific Phosphorylation of CXCR4 Is Dynamically Regulated by Multiple Kinases and Results in Differential Modulation of CXCR4 Signaling. J Biol Chem 285, 7805-17 20048153
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S321-p - CXCR4 (human)
Orthologous residues
CXCR4 (human): S321‑p, CXCR4 (mouse): S328‑p, CXCR4 (rat): S318‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SDF-1alpha increase

S324-p - CXCR4 (human)
Orthologous residues
CXCR4 (human): S324‑p, CXCR4 (mouse): S331‑p, CXCR4 (rat): S321‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), astrocyte
 Cellular systems studied:  cell lines, primary cultured cells
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCD (human) pharmacological inhibitor of upstream enzyme, genetic knockout/knockin of upstream enzyme
KINASE GRK6 (human) genetic knockout/knockin of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SDF-1alpha increase
peptide antagonist SDF-1alpha inhibit treatment-induced increase
bisindolylmaleimide SDF-1alpha inhibit treatment-induced increase
Bis V SDF-1alpha no effect upon treatment-induced increase
Go 6976 SDF-1alpha no effect upon treatment-induced increase
rottlerin SDF-1alpha inhibit treatment-induced increase

S325-p - CXCR4 (human)
Orthologous residues
CXCR4 (human): S325‑p, CXCR4 (mouse): S332‑p, CXCR4 (rat): S322‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), astrocyte
 Cellular systems studied:  cell lines, primary cultured cells
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GRK6 (human) genetic knockout/knockin of upstream enzyme
KINASE PKCD (human) pharmacological inhibitor of upstream enzyme, genetic knockout/knockin of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SDF-1alpha increase
peptide antagonist SDF-1alpha inhibit treatment-induced increase
bisindolylmaleimide SDF-1alpha inhibit treatment-induced increase
Bis V SDF-1alpha no effect upon treatment-induced increase
Go 6976 SDF-1alpha no effect upon treatment-induced increase
rottlerin SDF-1alpha inhibit treatment-induced increase

S330-p - CXCR4 (human)
Orthologous residues
CXCR4 (human): S330‑p, CXCR4 (mouse): S337‑p, CXCR4 (rat): S327‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), astrocyte
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GRK6 (mouse) electrophoretic mobility shift
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SDF-1alpha increase

S338-p - CXCR4 (human)
Orthologous residues
CXCR4 (human): S338‑p, CXCR4 (mouse): S345‑p, CXCR4 (rat): S335‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  S338, S339, or S341
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SDF-1alpha increase

S339-p - CXCR4 (human)
Orthologous residues
CXCR4 (human): S339‑p, CXCR4 (mouse): S346‑p, CXCR4 (rat): S336‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), astrocyte
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  S338, S339, or S341
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GRK6 (mouse) electrophoretic mobility shift
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SDF-1alpha increase
SDF-1alpha increase

S341-p - CXCR4 (human)
Orthologous residues
CXCR4 (human): S341‑p, CXCR4 (mouse): S348‑p, CXCR4 (rat): S338‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  S338, S339, or S341
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SDF-1alpha increase

S346-p - CXCR4 (human)
Orthologous residues
CXCR4 (human): S346‑p, CXCR4 (mouse): S353‑p, CXCR4 (rat): S343‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  S346, S347, or S348
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SDF-1alpha increase

S347-p - CXCR4 (human)
Orthologous residues
CXCR4 (human): S347‑p, CXCR4 (mouse): S354‑p, CXCR4 (rat): S344‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  S346, S347, or S348
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SDF-1alpha increase

S348-p - CXCR4 (human)
Orthologous residues
CXCR4 (human): S348‑p, CXCR4 (mouse): S355‑p, CXCR4 (rat): S345‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  S346, S347, or S348
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SDF-1alpha increase

S351-p - CXCR4 (human)
Orthologous residues
CXCR4 (human): S351‑p, CXCR4 (mouse): S358‑p, CXCR4 (rat): S348‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  S351 or S352
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SDF-1alpha increase

S352-p - CXCR4 (human)
Orthologous residues
CXCR4 (human): S352‑p, CXCR4 (mouse): S359‑p, CXCR4 (rat): S349‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  S351 or S352
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SDF-1alpha increase


Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.