Curated Information
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Curated Information Page
PubMed Id: 16484495 
Yin L, Wang J, Klein PS, Lazar MA (2006) Nuclear receptor Rev-erbalpha is a critical lithium-sensitive component of the circadian clock. Science 311, 1002-5 16484495
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S9-p - GSK3B (human)
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S3‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum withdrawal increase serum shock- 50% serum followed by 0.5% serum 2 hrs later
lithium increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited

S55-p - NR1D1 (human)
Orthologous residues
NR1D1 (human): S55‑p, NR1D1 (mouse): S55‑p, NR1D1 (rat): S55‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Disease tissue studied:  liver cancer
 Relevant cell lines - cell types - tissues:  293T (epithelial), HepG2 (hepatic)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3B (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) modification site within consensus motif, transfection of dominant-negative enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
lithium decrease
Downstream Regulation
 Effect of modification (function):  protein stabilization

S59-p - NR1D1 (human)
Orthologous residues
NR1D1 (human): S59‑p, NR1D1 (mouse): S59‑p, NR1D1 (rat): S59‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Disease tissue studied:  liver cancer
 Relevant cell lines - cell types - tissues:  293T (epithelial), HepG2 (hepatic)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3B (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) modification site within consensus motif, transfection of dominant-negative enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
lithium decrease
Downstream Regulation
 Effect of modification (function):  protein stabilization


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