Curated Information
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Curated Information Page
PubMed Id: 19713527 
Yang WL, et al. (2009) The E3 ligase TRAF6 regulates Akt ubiquitination and activation. Science 325, 1134-8 19713527
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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K8-ub - Akt1 (human)
Orthologous residues
Akt1 (human): K8‑ub, Akt1 (mouse): K8‑ub, Akt1 (rat): K8‑ub, Akt1 (fruit fly): K109‑ub, Akt1 (cow): K8‑ub
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE TRAF6 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE TRAF6 (human) transfection of inactive enzyme, transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase
serum increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, intracellular localization, phosphorylation
 Comments:  promotes Akt membrane recruitment and phosphorylation, resulting in activation

K14-ub - Akt1 (human)
Orthologous residues
Akt1 (human): K14‑ub, Akt1 (mouse): K14‑ub, Akt1 (rat): K14‑ub, Akt1 (fruit fly): K115‑ub, Akt1 (cow): K14‑ub
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE TRAF6 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE TRAF6 (human) transfection of inactive enzyme, transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase
serum increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, intracellular localization, phosphorylation
 Comments:  promotes Akt membrane recruitment and phosphorylation, resulting in activation

T308-p - Akt1 (human)
Orthologous residues
Akt1 (human): T308‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TRAF6 (human) increase
LPS, serum TRAF6 (human) increase
serum, IL-1b TRAF6 (human) increase
doxorubicin TRAF6 (human) increase
cisplatin TRAF6 (human) increase
IGF-1 TRAF6 (human) increase
serum TRAF6 (human) increase

S473-p - Akt1 (human)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 TRAF6 (human) increase
serum TRAF6 (human) increase


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