Curated Information
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Curated Information Page
PubMed Id: 17213202 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Cho YY, et al. (2007) RSK2 mediates muscle cell differentiation through regulation of NFAT3. J Biol Chem 282, 8380-92 17213202
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S168-p - NFAT3 (human) ▲
Orthologous residues
NFAT3 (human): S168‑p, NFAT3 iso11 (human): S231‑p, NFAT3 (mouse): S168‑p, NFAT3 (rat): S168‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Downstream Regulation
 Effect of modification (process):  cell differentiation, altered

S170-p - NFAT3 (human) ▲
Orthologous residues
NFAT3 (human): S170‑p, NFAT3 iso11 (human): S233‑p, NFAT3 (mouse): S170‑p, NFAT3 (rat): S170‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Downstream Regulation
 Effect of modification (process):  cell differentiation, altered

S281-p - NFAT3 (human) ▲
Orthologous residues
NFAT3 (human): S281‑p, NFAT3 iso11 (human): S344‑p, NFAT3 (mouse): S281‑p, NFAT3 (rat): S281‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast) [RSK2 (human), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE RSK2 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE RSK2 (human) mutation in upstream enzyme recognition motif, co-immunoprecipitation
Downstream Regulation
 Effect of modification (process):  transcription, altered

S285-p - NFAT3 (human) ▲
Orthologous residues
NFAT3 (human): S285‑p, NFAT3 iso11 (human): S348‑p, NFAT3 (mouse): S285‑p, NFAT3 (rat): S285‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast) [RSK2 (human), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE RSK2 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE RSK2 (human) mutation in upstream enzyme recognition motif, co-immunoprecipitation
Downstream Regulation
 Effect of modification (process):  transcription, altered

S289-p - NFAT3 (human) ▲
Orthologous residues
NFAT3 (human): S289‑p, NFAT3 iso11 (human): S352‑p, NFAT3 (mouse): S289‑p, NFAT3 (rat): S289‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast) [RSK2 (human), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE RSK2 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE RSK2 (human) mutation in upstream enzyme recognition motif, co-immunoprecipitation

S344-p - NFAT3 (human) ▲
Orthologous residues
NFAT3 (human): S344‑p, NFAT3 iso11 (human): S407‑p, NFAT3 (mouse): S344‑p, NFAT3 (rat): S344‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast) [RSK2 (human), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE RSK2 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE RSK2 (human) mutation in upstream enzyme recognition motif, co-immunoprecipitation


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