Curated Information
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Curated Information Page
PubMed Id: 18449195 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Richard DJ, et al. (2008) Single-stranded DNA-binding protein hSSB1 is critical for genomic stability. Nature 453, 677-81 18449195
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S1981-p - ATM (human) ▲
Orthologous residues
ATM (human): S1981‑p, ATM (mouse): S1987‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  fibroblast-foreskin
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation OBFC2B (human) increase OBFC2B/SSB1 siRNA reduces induction
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

S317-p - Chk1 (human) ▲
Orthologous residues
Chk1 (human): S317‑p, Chk1 (mouse): S317‑p, Chk1 (rat): S317‑p, Chk1 (chicken): S317‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  fibroblast-foreskin
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation OBFC2B (human) increase OBFC2B/SSB1 siRNA reduces induction

T68-p - Chk2 (human) ▲
Orthologous residues
Chk2 (human): T68‑p, Chk2 iso12 (human): T68‑p, Chk2 (mouse): T77‑p, Chk2 (rat): T76‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  fibroblast-foreskin
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation OBFC2B (human) increase OBFC2B/SSB1 siRNA reduces induction

S343-p - NBS1 (human) ▲
Orthologous residues
NBS1 (human): S343‑p, NBS1 (mouse): S343‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  fibroblast-foreskin
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation OBFC2B (human) increase OBFC2B/SSB1 siRNA reduces induction

T117-p - OBFC2B (human) ▲
Orthologous residues
OBFC2B (human): T117‑p, OBFC2B (mouse): T117‑p, OBFC2B (rat): T117‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  fibroblast-foreskin
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ATM (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation OBFC2B (human) increase OBFC2B/SSB1 siRNA reduces induction

S15-p - p53 (human) ▲
Orthologous residues
p53 (human): S15‑p, p53 (mouse): S15‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (monkey): S15‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  fibroblast-foreskin
 Cellular systems studied:  cell lines
 Species studied:  human


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