Curated Information
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Curated Information Page
PubMed Id: 8657113 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Lin R, et al. (1996) Phosphorylation of IkappaBalpha in the C-terminal PEST domain by casein kinase II affects intrinsic protein stability. Mol Cell Biol 16, 1401-9 8657113
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S283-p - IkB-alpha (human) ▲
Orthologous residues
IkB‑alpha (human): S283‑p, IkB‑alpha (mouse): S283‑p, IkB‑alpha (rat): S283‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CK2-A1 (human)
Downstream Regulation
 Effect of modification (function):  protein degradation
 Comments:  S283/T291/T299A triple mutant showed increased IkB-alpha stability.

T291-p - IkB-alpha (human) ▲
Orthologous residues
IkB‑alpha (human): T291‑p, IkB‑alpha (mouse): T291‑p, IkB‑alpha (rat): T291‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CK2-A1 (human)
Downstream Regulation
 Effect of modification (function):  protein degradation
 Comments:  S283/T291/T299A triple mutant showed increased IkB-alpha stability.

T299-p - IkB-alpha (human) ▲
Orthologous residues
IkB‑alpha (human): T299‑p, IkB‑alpha (mouse): T296‑p, IkB‑alpha (rat): T296‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CK2-A1 (human)
Downstream Regulation
 Effect of modification (function):  protein degradation
 Comments:  S283/T291/T299A triple mutant showed increased IkB-alpha stability.


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