Curated Information
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Curated Information Page
PubMed Id: 17906618 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Plun-Favreau H, et al. (2007) The mitochondrial protease HtrA2 is regulated by Parkinson's disease-associated kinase PINK1. Nat Cell Biol 9, 1243-1252 17906618
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S473-p - Akt1 (human)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  neuroblastoma
 Relevant cell lines - cell types - tissues:  'brain, caudate-putamen', 293 (epithelial), MEF (fibroblast), SH-SY5Y (neural crest)
 Cellular systems studied:  cell lines, tissue
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
4-HT increase

T202-p - ERK1 (human)
Orthologous residues
ERK1 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  neuroblastoma
 Relevant cell lines - cell types - tissues:  'brain, caudate-putamen', 293 (epithelial), MEF (fibroblast), SH-SY5Y (neural crest)
 Cellular systems studied:  cell lines, tissue
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
4-HT MKK3 (human) increase
4-HT ARAF (human) increase

Y204-p - ERK1 (human)
Orthologous residues
ERK1 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  neuroblastoma
 Relevant cell lines - cell types - tissues:  'brain, caudate-putamen', 293 (epithelial), MEF (fibroblast), SH-SY5Y (neural crest)
 Cellular systems studied:  cell lines, tissue
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
4-HT MKK3 (human) increase
4-HT ARAF (human) increase

T185-p - ERK2 (human)
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  neuroblastoma
 Relevant cell lines - cell types - tissues:  'brain, caudate-putamen', 293 (epithelial), MEF (fibroblast), SH-SY5Y (neural crest)
 Cellular systems studied:  cell lines, tissue
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
4-HT MKK3 (human) increase
4-HT ARAF (human) increase

Y187-p - ERK2 (human)
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  neuroblastoma
 Relevant cell lines - cell types - tissues:  'brain, caudate-putamen', 293 (epithelial), MEF (fibroblast), SH-SY5Y (neural crest)
 Cellular systems studied:  cell lines, tissue
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
4-HT MKK3 (human) increase
4-HT ARAF (human) increase

S142-p - HTRA2 (human)
Orthologous residues
HTRA2 (human): S142‑p, HTRA2 iso2 (human): S142‑p, HTRA2 (mouse): S142‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  neuroblastoma
 Relevant cell lines - cell types - tissues:  'brain, caudate-putamen', 293 (epithelial), MEF (fibroblast), SH-SY5Y (neural crest)
 Cellular systems studied:  cell lines, tissue
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE P38G (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PINK1 (human) increase PINK1 siRNA decreases
4-HT increase
4-HT MEKK3 (human) increase
4-HT PINK1 (human) increase
4-HT MKK3 (human), MKK6 (human) increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
Associated Diseases
Diseases: Alterations: Comments:
Parkinson's disease increased, decreased hyperphosphorylation in Parkinsons disease brains; hypophosphorylation in Parkinson's disease brains carrying C575R and Y431H mutations

S400-p - HTRA2 (human)
Orthologous residues
HTRA2 (human): S400‑p, HTRA2 iso2 (human): , HTRA2 (mouse): S400‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  neuroblastoma
 Relevant cell lines - cell types - tissues:  'brain, caudate-putamen', 293 (epithelial), MEF (fibroblast), SH-SY5Y (neural crest)
 Cellular systems studied:  cell lines, tissue
 Species studied:  human

T183-p - JNK1 (human)
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  neuroblastoma
 Relevant cell lines - cell types - tissues:  'brain, caudate-putamen', 293 (epithelial), MEF (fibroblast), SH-SY5Y (neural crest)
 Cellular systems studied:  cell lines, tissue
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
4-HT MKK3 (human) increase
4-HT ARAF (human) increase

Y185-p - JNK1 (human)
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  neuroblastoma
 Relevant cell lines - cell types - tissues:  'brain, caudate-putamen', 293 (epithelial), MEF (fibroblast), SH-SY5Y (neural crest)
 Cellular systems studied:  cell lines, tissue
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
4-HT MKK3 (human) increase
4-HT ARAF (human) increase

T180-p - P38A (human)
Orthologous residues
P38A (human): T180‑p, P38A iso2 (human): T180‑p, P38A (mouse): T180‑p, P38A iso3 (mouse): T180‑p, P38A (rat): T180‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  neuroblastoma
 Relevant cell lines - cell types - tissues:  'brain, caudate-putamen', 293 (epithelial), MEF (fibroblast), SH-SY5Y (neural crest)
 Cellular systems studied:  cell lines, tissue
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
4-HT MKK3 (human) increase
4-HT ARAF (human) increase

Y182-p - P38A (human)
Orthologous residues
P38A (human): Y182‑p, P38A iso2 (human): Y182‑p, P38A (mouse): Y182‑p, P38A iso3 (mouse): Y182‑p, P38A (rat): Y182‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  neuroblastoma
 Relevant cell lines - cell types - tissues:  'brain, caudate-putamen', 293 (epithelial), MEF (fibroblast), SH-SY5Y (neural crest)
 Cellular systems studied:  cell lines, tissue
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
4-HT MKK3 (human) increase
4-HT ARAF (human) increase


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