Curated Information
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Curated Information Page
PubMed Id: 17715136 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Lee IH, et al. (2007) Akt mediates the effect of insulin on epithelial sodium channels by inhibiting Nedd4-2. J Biol Chem 282, 29866-73 17715136
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S342-p - NEDD4L (human)
Orthologous residues
NEDD4L (human): S342‑p, NEDD4L iso5 (human): S342‑p, NEDD4L (mouse): S371‑p, NEDD4L iso3 (mouse): S371‑p, NEDD4L (rat): S330‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  FRTL-5 (thyroid cell)
 Cellular systems studied:  cell lines
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Akt1 (human) increase Akt2 and Akt3 siRNAs also inhibited ENaC activity, perhaps via NEDD4L phosphorylation.
SGK1 (human) increase
Downstream Regulation
 Effect of modification (function):  activity, inhibited
 Comments:  Phosphorylation of S342 and S428 (more important site) inhibits Nedd4L which regulates ENaC activity.

S428-p - NEDD4L (human)
Orthologous residues
NEDD4L (human): S428‑p, NEDD4L iso5 (human): S408‑p, NEDD4L (mouse): S457‑p, NEDD4L iso3 (mouse): S437‑p, NEDD4L (rat): S416‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  FRTL-5 (thyroid cell)
 Cellular systems studied:  cell lines
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Akt1 (human) increase Akt2 and Akt3 siRNAs also inhibited ENaC activity, perhaps via NEDD4L phosphorylation.
SGK1 (human) increase
Downstream Regulation
 Effect of modification (function):  activity, inhibited
 Comments:  Phosphorylation of S342 and S428 (more important site) inhibits Nedd4L which regulates ENaC activity.


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