Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Curated Information Page
PubMed Id: 17870612 
Liang X, et al. (2007) Quantitative Comparison of IMAC and TiO(2) Surfaces Used in the Study of Regulated, Dynamic Protein Phosphorylation. J Am Soc Mass Spectrom 18, 1932-44 17870612
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

Y827-p - Ack (human)
Orthologous residues
Ack (human): Y827‑p, Ack iso3 (human): Y905‑p, Ack (mouse): Y842‑p, Ack iso2 (mouse): Y827‑p, Ack (rat): Y827‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y656-p - ARHGEF5 (human)
Orthologous residues
ARHGEF5 (human): Y656‑p, ARHGEF5 (mouse): G642‑p, ARHGEF5 (rat): G650‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S1126-p - ARHGEF5 (human)
Orthologous residues
ARHGEF5 (human): S1126‑p, ARHGEF5 (mouse): S1110‑p, ARHGEF5 (rat): S1118‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S518-p - ARHGEF7 (human)
Orthologous residues
ARHGEF7 (human): S518‑p, ARHGEF7 iso1 (human): S340‑p, ARHGEF7 iso7 (human): S496‑p, ARHGEF7 (mouse): S497‑p, ARHGEF7 iso2 (mouse): S497‑p, ARHGEF7 iso3 (mouse): S497‑p, ARHGEF7 (rat): S340‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S703-p - ARHGEF7 (human)
Orthologous residues
ARHGEF7 (human): S703‑p, ARHGEF7 iso1 (human): S525‑p, ARHGEF7 iso7 (human): , ARHGEF7 (mouse): S682‑p, ARHGEF7 iso2 (mouse): S682‑p, ARHGEF7 iso3 (mouse): S607‑p, ARHGEF7 (rat): S525‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S658-p - BCLAF1 (human)
Orthologous residues
BCLAF1 (human): S658‑p, BCLAF1 iso3 (human): S656‑p, BCLAF1 iso4 (human): S485‑p, BCLAF1 (mouse): S656‑p, BCLAF1 (rat): S658‑p, BCLAF1 iso3 (rat):
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y737-p - CENTD2 (human)
Orthologous residues
CENTD2 (human): Y737‑p, CENTD2 iso1 (human): Y497‑p, CENTD2 iso5 (human): , CENTD2 (mouse): Y739‑p, CENTD2 (rat): Y741‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y421-p - Cortactin (human)
Orthologous residues
Cortactin (human): Y421‑p, Cortactin iso3 (human): Y384‑p, Cortactin (mouse): Y421‑p, Cortactin (rat): Y383‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

T693-p - EGFR (human)
Orthologous residues
EGFR (human): T693‑p, EGFR iso5 (human): T693‑p, EGFR (mouse): T695‑p, EGFR (rat): T694‑p, EGFR (pig): T693‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y998-p - EGFR (human)
Orthologous residues
EGFR (human): Y998‑p, EGFR iso5 (human): Y998‑p, EGFR (mouse): Y1000‑p, EGFR (rat): Y999‑p, EGFR (pig): Y998‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y1016-p - EGFR (human)
Orthologous residues
EGFR (human): Y1016‑p, EGFR iso5 (human): Y1016‑p, EGFR (mouse): Y1018‑p, EGFR (rat): Y1017‑p, EGFR (pig): Y1016‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S1070-p - EGFR (human)
Orthologous residues
EGFR (human): S1070‑p, EGFR iso5 (human): S1070‑p, EGFR (mouse): S1070‑p, EGFR (rat): S1069‑p, EGFR (pig): S1069‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y1092-p - EGFR (human)
Orthologous residues
EGFR (human): Y1092‑p, EGFR iso5 (human): , EGFR (mouse): Y1092‑p, EGFR (rat): Y1091‑p, EGFR (pig): Y1091‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y1172-p - EGFR (human)
Orthologous residues
EGFR (human): Y1172‑p, EGFR iso5 (human): , EGFR (mouse): Y1172‑p, EGFR (rat): Y1171‑p, EGFR (pig): Y1171‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y1197-p - EGFR (human)
Orthologous residues
EGFR (human): Y1197‑p, EGFR iso5 (human): , EGFR (mouse): Y1197‑p, EGFR (rat): Y1196‑p, EGFR (pig): Y1196‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y575-p - EphA2 (human)
Orthologous residues
EphA2 (human): Y575‑p, EphA2 (mouse): R576‑p, EphA2 (rat): Y576‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y772-p - EphA2 (human)
Orthologous residues
EphA2 (human): Y772‑p, EphA2 (mouse): Y773‑p, EphA2 (rat): Y773‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y186-p - epsin 2 (human)
Orthologous residues
epsin 2 (human): Y186‑p, epsin 2 iso2 (human): Y186‑p, epsin 2 (mouse): Y186‑p, epsin 2 iso3 (mouse): Y186‑p, epsin 2 (rat): Y186‑p, epsin 2 iso1 (rat): Y186‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y659-p - Gab1 (human)
Orthologous residues
Gab1 (human): Y659‑p, Gab1 iso2 (human): Y689‑p, Gab1 (mouse): Y660‑p, Gab1 (rat): Y658‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S1054-p - HER2 (human)
Orthologous residues
HER2 (human): S1054‑p, HER2 iso2 (human): S444‑p, HER2 (mouse): S1055‑p, HER2 (rat): S1056‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y1248-p - HER2 (human)
Orthologous residues
HER2 (human): Y1248‑p, HER2 iso2 (human): Y638‑p, HER2 (mouse): Y1249‑p, HER2 (rat): Y1250‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y228-p - PIK3C2B (human)
Orthologous residues
PIK3C2B (human): Y228‑p, PIK3C2B (mouse): Y227‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y771-p - PLCG1 (human)
Orthologous residues
PLCG1 (human): Y771‑p, PLCG1 iso2 (human): Y771‑p, PLCG1 (mouse): Y771‑p, PLCG1 (rat): Y771‑p, PLCG1 (cow): Y771‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y1253-p - PLCG1 (human)
Orthologous residues
PLCG1 (human): Y1253‑p, PLCG1 iso2 (human): Y1254‑p, PLCG1 (mouse): Y1253‑p, PLCG1 (rat): Y1253‑p, PLCG1 (cow): Y1254‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y88-p - PXN iso2 (human)
Orthologous residues
PXN (human): Y88‑p, PXN iso2 (human): Y88‑p, PXN iso3 (human): Y88‑p, PXN iso6 (human): Y95‑p, PXN (mouse): Y88‑p, PXN iso2 (mouse): Y88‑p, PXN (rat): Y88‑p, PXN (chicken): Y88‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y118-p - PXN iso2 (human)
Orthologous residues
PXN (human): Y118‑p, PXN iso2 (human): Y118‑p, PXN iso3 (human): Y118‑p, PXN iso6 (human): Y125‑p, PXN (mouse): Y118‑p, PXN iso2 (mouse): Y118‑p, PXN (rat): Y118‑p, PXN (chicken): Y118‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y427-p - Shc1 (human)
Orthologous residues
Shc1 (human): Y427‑p, Shc1 iso2 (human): Y317‑p, Shc1 iso3 (human): Y272‑p, Shc1 iso6 (human): Y428‑p, Shc1 iso7 (human): Y318‑p, Shc1 (mouse): Y423‑p, Shc1 iso2 (mouse): Y313‑p, Shc1 iso3 (mouse): Y268‑p, Shc1 (rat): Y423‑p, Shc1 iso2 (rat): Y313‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y1135-p - SHIP-2 (human)
Orthologous residues
SHIP‑2 (human): Y1135‑p, SHIP‑2 (mouse): Y1136‑p, SHIP‑2 (rat): Y1136‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y699-p - STAT5B (human)
Orthologous residues
STAT5B (human): Y699‑p, STAT5B (mouse): Y699‑p, STAT5B (rat): Y699‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S243-p - Trap150 (human)
Orthologous residues
Trap150 (human): S243‑p, Trap150 (mouse): S243‑p, Trap150 (rat): S243‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S682-p - Trap150 (human)
Orthologous residues
Trap150 (human): S682‑p, Trap150 (mouse): S679‑p, Trap150 (rat): S679‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase


Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.