Curated Information
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Curated Information Page
PubMed Id: 10734067 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Sakaguchi K, et al. (2000) Damage-mediated phosphorylation of human p53 threonine 18 through a cascade mediated by a casein 1-like kinase. Effect on Mdm2 binding. J Biol Chem 275, 9278-83 10734067
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S15-p - p53 (human)
Orthologous residues
p53 (human): S15‑p, p53 (mouse): S15‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (monkey): S15‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  A549 (pulmonary), fibroblast, NCI-H1299 (pulmonary), WS1 (fibroblast)
 Cellular systems studied:  cell lines, primary cultured cells
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE DNAPK (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Comments:  phosphorylation of S15 is required for phosphorylation of T18

T18-p - p53 (human)
Orthologous residues
p53 (human): T18‑p, p53 (mouse): T18‑p, p53 iso2 (mouse): T21‑p, p53 (rat): T18‑p, p53 (rabbit): T18‑p, p53 (monkey): T18‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  A549 (pulmonary), fibroblast, NCI-H1299 (pulmonary), WS1 (fibroblast)
 Cellular systems studied:  cell lines, primary cultured cells
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK1D (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
MDM2 (human) Disrupts FRET

S20-p - p53 (human)
Orthologous residues
p53 (human): S20‑p, p53 (mouse): S20‑p, p53 iso2 (mouse): S23‑p, p53 (rat): S20‑p, p53 (rabbit): S20‑p, p53 (monkey): S20‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  A549 (pulmonary), fibroblast, NCI-H1299 (pulmonary), WS1 (fibroblast)
 Cellular systems studied:  cell lines, primary cultured cells
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase


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