Curated Information
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Curated Information Page
PubMed Id: 17640984 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Luo M, et al. (2007) Phosphorylation of human insulin receptor substrate-1 at Serine 629 plays a positive role in insulin signaling. Endocrinology 148, 4895-905 17640984
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S473-p - Akt1 (human)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p

S616-p - IRS1 (human)
Orthologous residues
IRS1 (human): S616‑p, IRS1 (mouse): S612‑p, IRS1 (rat): S612‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast) [IRS1 (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  hamster

S629-p - IRS1 (human)
Orthologous residues
IRS1 (human): S629‑p, IRS1 (mouse): N625‑p, IRS1 (rat): N625‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast) [IRS1 (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  hamster
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) transfection of dominant-negative enzyme, transfection of wild-type enzyme, transfection of constitutively active enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
Akt-I-1 insulin inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PIK3R1 (human) Induces co-immunoprecipitation

Y632-p - IRS1 (human)
Orthologous residues
IRS1 (human): Y632‑p, IRS1 (mouse): Y628‑p, IRS1 (rat): Y628‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast) [IRS1 (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  hamster

S636-p - IRS1 (human)
Orthologous residues
IRS1 (human): S636‑p, IRS1 (mouse): S632‑p, IRS1 (rat): S632‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast) [IRS1 (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  hamster
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
 Comments:  Phosphorylation of S629 by Akt decreases ERK phosphorylation of S636/S629.

S639-p - IRS1 (human)
Orthologous residues
IRS1 (human): S639‑p, IRS1 (mouse): S635‑p, IRS1 (rat): S635‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast) [IRS1 (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  hamster
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
 Comments:  Phosphorylation of S629 by Akt decreases ERK phosphorylation of S636/S629.


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