Xirodimas DP, et al. (2004) Mdm2-mediated NEDD8 conjugation of p53 inhibits its transcriptional activity. Cell 118, 83-97 15242646

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K370-ne - p53 (human) ▲

Modsite: RAHssHLkskkGQst SwissProt Entrez-Gene Orthologous residues p53 (human): K370‑ne, p53 iso2 (human): ‑, p53 iso4 (human): K331‑ne, p53 (mouse): K367‑ne, p53 iso2 (mouse): ‑, p53 (rat): K368‑ne, p53 (rabbit): K368‑ne, p53 (green monkey): K370‑ne Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Disease tissue studied:  lung cancer, non-small cell lung cancer Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme UBIQUITIN LIGASE MDM2 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE MDM2 (human) genetic knockout/knockin of upstream enzyme, siRNA inhibition of enzyme Downstream Regulation Effect of modification (process):  transcription, inhibited

K372-ne - p53 (human) ▲

Modsite: HssHLkskkGQstsR SwissProt Entrez-Gene Orthologous residues p53 (human): K372‑ne, p53 iso2 (human): ‑, p53 iso4 (human): K333‑ne, p53 (mouse): K369‑ne, p53 iso2 (mouse): ‑, p53 (rat): K370‑ne, p53 (rabbit): K370‑ne, p53 (green monkey): K372‑ne Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Disease tissue studied:  lung cancer, non-small cell lung cancer Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme UBIQUITIN LIGASE MDM2 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE MDM2 (human) genetic knockout/knockin of upstream enzyme, siRNA inhibition of enzyme Downstream Regulation Effect of modification (process):  transcription, inhibited

K373-ne - p53 (human) ▲

Modsite: ssHLkskkGQstsRH SwissProt Entrez-Gene Orthologous residues p53 (human): K373‑ne, p53 iso2 (human): ‑, p53 iso4 (human): K334‑ne, p53 (mouse): K370‑ne, p53 iso2 (mouse): ‑, p53 (rat): K371‑ne, p53 (rabbit): K371‑ne, p53 (green monkey): K373‑ne Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Disease tissue studied:  lung cancer, non-small cell lung cancer Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme UBIQUITIN LIGASE MDM2 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE MDM2 (human) genetic knockout/knockin of upstream enzyme, siRNA inhibition of enzyme Downstream Regulation Effect of modification (process):  transcription, inhibited