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Orthologous residues
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NPM1 (human): T199‑p, NPM1 iso2 (human): ‑, NPM1 iso3 (human): T199‑p, NPM1 (mouse): T198‑p, NPM1 (rat): T198‑p
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Characterization
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Methods used to characterize site in vivo:
phospho-antibody, western blotting
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Relevant cell lines - cell types - tissues:
fibroblast-embryo [CDK2 (mouse), homozygous knockout], fibroblast-embryo [CDK4 (mouse), homozygous knockout], fibroblast-embryo [p53 (mouse), homozygous knockout]
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Cellular systems studied:
primary cultured cells
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Species studied:
mouse
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Upstream Regulation
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Treatments, proteins and their effect on site modification:
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Treatments
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Referenced Treatments
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Manipulated Protein
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Referenced Protein
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Effect
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Notes
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|
serum
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increase
|
wild-type
|
|
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serum
|
CDK4 (mouse)
|
|
no change compared to control
|
Cdk4 -/-
|
|
|
serum
|
CDK2 (mouse)
|
|
augment treatment-induced increase
|
Cdk2 -/- decrease
|
|
|
|
CDK2 (mouse)
|
|
increase
|
Cdk2 -/- decrease
|
|
|
|
p53 (mouse)
|
|
decrease
|
p53-/- increase
|
|
|
|
CDK4 (mouse)
|
|
no effect upon treatment-induced increase
|
cdk4 -/- no effect
|
|
|
Downstream Regulation
|
|
Effect of modification (process):
cytoskeletal reorganization
|
|
Comments:
centrosome duplication
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