Curated Information
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Curated Information Page
PubMed Id: 19935711 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Treins C, et al. (2010) Rictor is a novel target of p70 S6 kinase-1. Oncogene 29, 1003-16 19935711
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S473-p - Akt1 (human)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PI-103 decrease
LY294002 decrease
rapamycin no change compared to control
insulin increase
rapamycin insulin no effect upon treatment-induced increase
amino acids no change compared to control

T412-p - p70S6K (human)
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PI-103 decrease
LY294002 decrease
rapamycin decrease
amino acids increase
rapamycin amino acids inhibit treatment-induced increase
insulin increase
rapamycin insulin inhibit treatment-induced increase

S21-p - Rictor (human)
Orthologous residues
Rictor (human): S21‑p, Rictor (mouse): S21‑p, Rictor (rat): S6‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S1035-p - Rictor (human)
Orthologous residues
Rictor (human): S1035‑p, Rictor (mouse): S1035‑p, Rictor (rat): S1020‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

T1135-p - Rictor (human)
Orthologous residues
Rictor (human): T1135‑p, Rictor (mouse): T1135‑p, Rictor (rat): T1120‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE p70S6K (human)
KINASE P70S6KB (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE p70S6K (human) genetic knockout/knockin of upstream enzyme, siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PI-103 decrease
LY294002 decrease
rapamycin decrease
amino acids increase
rapamycin amino acids inhibit treatment-induced increase
insulin increase
rapamycin insulin inhibit treatment-induced increase
EGF increase
serum increase
calyculin A increase
U0126 no change compared to control
mTOR (human) increase mTOR siRNA decreases
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 epsilon (human) Induces co-immunoprecipitation

S1138-p - Rictor (human)
Orthologous residues
Rictor (human): S1138‑p, Rictor (mouse): S1138‑p, Rictor (rat): S1123‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Comments:  ambiguous

T1172-p - Rictor (human)
Orthologous residues
Rictor (human): T1172‑p, Rictor (mouse): A1171‑p, Rictor (rat): T1157‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

S1174-p - Rictor (human)
Orthologous residues
Rictor (human): S1174‑p, Rictor (mouse): S1173‑p, Rictor (rat): S1159‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

T1175-p - Rictor (human)
Orthologous residues
Rictor (human): T1175‑p, Rictor (mouse): T1174‑p, Rictor (rat): T1160‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

S1177-p - Rictor (human)
Orthologous residues
Rictor (human): S1177‑p, Rictor (mouse): S1176‑p, Rictor (rat): S1162‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S1219-p - Rictor (human)
Orthologous residues
Rictor (human): S1219‑p, Rictor (mouse): S1218‑p, Rictor (rat): S1204‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

T1222-p - Rictor (human)
Orthologous residues
Rictor (human): T1222‑p, Rictor (mouse): T1221‑p, Rictor (rat): T1207‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

T1224-p - Rictor (human)
Orthologous residues
Rictor (human): T1224‑p, Rictor (mouse): T1223‑p, Rictor (rat): T1209‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

T1225-p - Rictor (human)
Orthologous residues
Rictor (human): T1225‑p, Rictor (mouse): T1224‑p, Rictor (rat): T1210‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

T1226-p - Rictor (human)
Orthologous residues
Rictor (human): T1226‑p, Rictor (mouse): T1225‑p, Rictor (rat): T1211‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

S1227-p - Rictor (human)
Orthologous residues
Rictor (human): S1227‑p, Rictor (mouse): S1226‑p, Rictor (rat): S1212‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

S1230-p - Rictor (human)
Orthologous residues
Rictor (human): S1230‑p, Rictor (mouse): S1229‑p, Rictor (rat): S1215‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

S1231-p - Rictor (human)
Orthologous residues
Rictor (human): S1231‑p, Rictor (mouse): S1230‑p, Rictor (rat): S1216‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

S1235-p - Rictor (human)
Orthologous residues
Rictor (human): S1235‑p, Rictor (mouse): S1234‑p, Rictor (rat): S1220‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S1292-p - Rictor (human)
Orthologous residues
Rictor (human): S1292‑p, Rictor (mouse): S1291‑p, Rictor (rat): S1277‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

S1293-p - Rictor (human)
Orthologous residues
Rictor (human): S1293‑p, Rictor (mouse): S1292‑p, Rictor (rat): S1278‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

T1295-p - Rictor (human)
Orthologous residues
Rictor (human): T1295‑p, Rictor (mouse): T1294‑p, Rictor (rat): T1280‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

S1302-p - Rictor (human)
Orthologous residues
Rictor (human): S1302‑p, Rictor (mouse): S1301‑p, Rictor (rat): S1287‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S1385-p - Rictor (human)
Orthologous residues
Rictor (human): S1385‑p, Rictor (mouse): S1384‑p, Rictor (rat): S1370‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

Y1386-p - Rictor (human)
Orthologous residues
Rictor (human): Y1386‑p, Rictor (mouse): Y1385‑p, Rictor (rat): Y1371‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

S1388-p - Rictor (human)
Orthologous residues
Rictor (human): S1388‑p, Rictor (mouse): S1387‑p, Rictor (rat): S1373‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

S1414-p - Rictor (human)
Orthologous residues
Rictor (human): S1414‑p, Rictor (mouse): S1413‑p, Rictor (rat): S1399‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

S1415-p - Rictor (human)
Orthologous residues
Rictor (human): S1415‑p, Rictor (mouse): S1414‑p, Rictor (rat): S1400‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

T1417-p - Rictor (human)
Orthologous residues
Rictor (human): T1417‑p, Rictor (mouse): T1416‑p, Rictor (rat): T1402‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

Y1418-p - Rictor (human)
Orthologous residues
Rictor (human): Y1418‑p, Rictor (mouse): Y1417‑p, Rictor (rat): Y1403‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

S1421-p - Rictor (human)
Orthologous residues
Rictor (human): S1421‑p, Rictor (mouse): S1420‑p, Rictor (rat): S1406‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

S1470-p - Rictor (human)
Orthologous residues
Rictor (human): S1470‑p, Rictor (mouse): S1469‑p, Rictor (rat): S1455‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

T1472-p - Rictor (human)
Orthologous residues
Rictor (human): T1472‑p, Rictor (mouse): A1471‑p, Rictor (rat): T1457‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

S1479-p - Rictor (human)
Orthologous residues
Rictor (human): S1479‑p, Rictor (mouse): S1478‑p, Rictor (rat): S1464‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S1591-p - Rictor (human)
Orthologous residues
Rictor (human): S1591‑p, Rictor (mouse): S1591‑p, Rictor (rat): S1577‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

T1592-p - Rictor (human)
Orthologous residues
Rictor (human): T1592‑p, Rictor (mouse): T1592‑p, Rictor (rat): T1578‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  ambiguous

S235-p - S6 (human)
Orthologous residues
S6 (human): S235‑p, S6 (mouse): S235‑p, S6 (rat): S235‑p, S6 (fruit fly): A234‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PI-103 decrease
LY294002 decrease
rapamycin decrease
amino acids increase
rapamycin amino acids inhibit treatment-induced increase
insulin increase
rapamycin insulin inhibit treatment-induced increase
increase Rictor wild-type
rapamycin inhibit treatment-induced increase

S236-p - S6 (human)
Orthologous residues
S6 (human): S236‑p, S6 (mouse): S236‑p, S6 (rat): S236‑p, S6 (fruit fly): S235‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PI-103 decrease
LY294002 decrease
rapamycin decrease
amino acids increase
rapamycin amino acids inhibit treatment-induced increase
insulin increase
rapamycin insulin inhibit treatment-induced increase
increase Rictor wild-type
rapamycin inhibit treatment-induced increase

T308-p - Akt1 (mouse)
Orthologous residues
Akt1 (human): T308‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum Rictor (human) increase Rictor +/+ MEFs
serum Rictor (mouse) decrease Rictor -/- increases
no change compared to control rictor wild-type
rapamycin increase

T450-p - Akt1 (mouse)
Orthologous residues
Akt1 (human): T450‑p, Akt1 (mouse): T450‑p, Akt1 (rat): T450‑p, Akt1 (fruit fly): T565‑p, Akt1 (cow): T450‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum Rictor (human) no change compared to control Rictor +/+ MEFs
serum Rictor (human) no change compared to control Rictor -/- no change
increase Rictor wild-type
rapamycin inhibit treatment-induced increase

S473-p - Akt1 (mouse)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum Rictor (human) increase Rictor +/+ MEFs
serum Rictor (human) no change compared to control Rictor -/- no change
no change compared to control rictor wild-type
rapamycin increase

T32-p - FOXO3A (mouse)
Orthologous residues
FOXO3A (human): T32‑p, FOXO3A (mouse): T32‑p, FOXO3A (rat): T32‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum Rictor (mouse) decrease Rictor -/- increases

S21-p - GSK3A (mouse)
Orthologous residues
GSK3A (human): S21‑p, GSK3A (mouse): S21‑p, GSK3A (rat): S21‑p, GSK3A (cow): S21‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum Rictor (human) increase Rictor +/+ MEFs
serum Rictor (mouse) decrease Rictor -/- increases

S9-p - GSK3B (mouse)
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S3‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum Rictor (human) increase Rictor +/+ MEFs
serum Rictor (mouse) decrease Rictor -/- increases

T346-p - NDRG1 (mouse)
Orthologous residues
NDRG1 (human): T346‑p, NDRG1 (mouse): T346‑p, NDRG1 (rat): T346‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum Rictor (human) increase Rictor +/+ MEFs
serum Rictor (human) no change compared to control Rictor -/- no change
increase Rictor wild-type
rapamycin inhibit treatment-induced increase
no change compared to control rictor wild-type
rapamycin increase

T412-p - p70S6K (mouse)
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum Rictor (human) increase Rictor +/+ MEFs
serum Rictor (mouse) decrease Rictor -/- increases

T638-p - PKCA (mouse)
Orthologous residues
PKCA (human): T638‑p, PKCA (mouse): T638‑p, PKCA (rat): T638‑p, PKCA (cow): T638‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum Rictor (human) no change compared to control Rictor +/+ MEFs
serum Rictor (human) no change compared to control Rictor -/- no change
increase rictor wild-type
rapamycin no effect upon treatment-induced increase

Y658-p - PKCA (mouse)
Orthologous residues
PKCA (human): Y658‑p, PKCA (mouse): Y658‑p, PKCA (rat): Y658‑p, PKCA (cow): Y658‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum Rictor (human) no change compared to control Rictor +/+ MEFs
serum Rictor (human) no change compared to control Rictor -/- no change
increase rictor wild-type
rapamycin no effect upon treatment-induced increase

T609-p - PKCB (mouse)
Orthologous residues
PKCB (human): T642‑p, PKCB iso2 (human): T641‑p, PKCB (mouse): T609‑p, PKCB iso2 (mouse): T641‑p, PKCB (rat): T642‑p, PKCB iso2 (rat): T641‑p, PKCB (cow): T641‑p
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum Rictor (human) no change compared to control Rictor -/- no change
increase rictor wild-type
rapamycin no effect upon treatment-induced increase

T1465-p - TSC2 (mouse)
Orthologous residues
TSC2 (human): T1462‑p, TSC2 iso3 (human): T1418‑p, TSC2 iso4 (human): T1439‑p, TSC2 (mouse): T1465‑p, TSC2 iso6 (mouse): T1443‑p, TSC2 (rat): T1466‑p, TSC2 iso2 (rat): T1423‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum Rictor (human) increase Rictor +/+ MEFs
serum Rictor (mouse) decrease Rictor -/- increases


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