|
Orthologous residues
|
|
zyxin (human): S142‑p, zyxin (mouse): C143‑p, zyxin (rat): S143‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
mutation of modification site
|
|
Relevant cell lines - cell types - tissues:
293 (epithelial)
|
|
Cellular systems studied:
cell lines
|
|
Enzymes shown to modify site in vitro:
|
|
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
Akt1 (human)
|
transfection of constitutively active enzyme, transfection of inactive enzyme
|
|
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
EGF
|
|
|
|
increase
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
intracellular localization, molecular association, regulation
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
14-3-3 gamma (human)
|
|
Induces
|
protein stabilization
|
|
co-immunoprecipitation
|
|
acinus (human)
|
|
Induces
|
protein stabilization
|
|
co-immunoprecipitation
|
|
|
Comments:
phosphorylation does not protect zyxin from proteolytic cleavage by caspase, but inhibits acinus degradation and suppresses apoptosis
|
