Curated Information
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Curated Information Page
PubMed Id: 17620057 
Wong S, Weber JD (2007) Deacetylation of the retinoblastoma tumour suppressor protein by SIRT1. Biochem J 407, 451-60 17620057
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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K382-ac - p53 (human)
Orthologous residues
p53 (human): K382‑ac, p53 (mouse): K376‑ac, p53 iso2 (mouse): , p53 (rat): K380‑ac, p53 (rabbit): K380‑ac, p53 (monkey): K382‑ac
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
DEACETYLASE SIRT1 (human)
ACETYLTRANSFERASE p300 (human)

K873-ac - Rb (human)
Orthologous residues
Rb (human): K873‑ac, Rb (mouse): K866‑ac, Rb (rat): K865‑ac
Characterization
 Methods used to characterize site in vivo immunoprecipitation, modification-specific antibody, western blotting
 Relevant cell lines - cell types - tissues:  BJT [SIRT1 (human), transfection]
 Cellular systems studied:  cell lines
 Enzymes shown to modify site in vitro
Type Enzyme
DEACETYLASE SIRT1 (human)
ACETYLTRANSFERASE p300 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEACETYLASE SIRT1 (human) siRNA inhibition of enzyme, co-immunoprecipitation, transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nicotinamide increase
siRNA nicotinamide augment treatment-induced increase SirT1 siRNA
siRNA increase SirT1 siRNA
etoposide increase
nicotinamide etoposide augment treatment-induced increase

K874-ac - Rb (human)
Orthologous residues
Rb (human): K874‑ac, Rb (mouse): K867‑ac, Rb (rat): K866‑ac
Characterization
 Methods used to characterize site in vivo immunoprecipitation, modification-specific antibody, western blotting
 Relevant cell lines - cell types - tissues:  BJT [SIRT1 (human), transfection]
 Cellular systems studied:  cell lines
 Enzymes shown to modify site in vitro
Type Enzyme
DEACETYLASE SIRT1 (human)
ACETYLTRANSFERASE p300 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEACETYLASE SIRT1 (human) siRNA inhibition of enzyme, co-immunoprecipitation, transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nicotinamide increase
siRNA nicotinamide augment treatment-induced increase SirT1 siRNA
siRNA increase SirT1 siRNA
etoposide increase
nicotinamide etoposide augment treatment-induced increase


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