Curated Information
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Curated Information Page
PubMed Id: 11278523 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
El Kouhen R, et al. (2001) Phosphorylation of Ser363, Thr370, and Ser375 residues within the carboxyl tail differentially regulates mu-opioid receptor internalization. J Biol Chem 276, 12774-80 11278523
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S363-p - MOR-1 (rat) ▲
Orthologous residues
MOR‑1 (human): S365‑p, MOR‑1 iso5 (human): S365‑p, MOR‑1 (mouse): S363‑p, MOR‑1 (rat): S363‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DAMGO increase
Downstream Regulation
 Effect of modification (function):  receptor internalization, altered
 Comments:  Mutation of Ser363 and Thr370 to Ala accelerated MOR internalization kinetics.

T370-p - MOR-1 (rat) ▲
Orthologous residues
MOR‑1 (human): T372‑p, MOR‑1 iso5 (human): T372‑p, MOR‑1 (mouse): T370‑p, MOR‑1 (rat): T370‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DAMGO increase
Downstream Regulation
 Effect of modification (function):  receptor internalization, altered
 Comments:  Mutation of Ser363 and Thr370 to Ala accelerated MOR internalization kinetics.

S375-p - MOR-1 (rat) ▲
Orthologous residues
MOR‑1 (human): S377‑p, MOR‑1 iso5 (human): S377‑p, MOR‑1 (mouse): S375‑p, MOR‑1 (rat): S375‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DAMGO increase
Downstream Regulation
 Effect of modification (function):  receptor internalization, altered
 Comments:  Mutation of Ser375 to Ala reduced the rate and extent of receptor internalization.


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