Curated Information
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Curated Information Page
PubMed Id: 17525164 
Um JH, et al. (2007) Activation of 5'-AMP-activated kinase with diabetes drug metformin induces casein kinase Iepsilon (CKIepsilon)-dependent degradation of clock protein mPer2. J Biol Chem 282, 20794-8 17525164
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S389-p - CK1E (human)
Orthologous residues
CK1E (human): S389‑p, CK1E (mouse): S389‑p, CK1E (rat): S389‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE AMPKA1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AMPKA1 (mouse) modification site within consensus motif
Downstream Regulation
 Effect of modification (function):  protein degradation
 Effect of modification (process):  transcription, altered
 Comments:  phaosphorylation induces degredation of mPer2 and expression of circadian clock genes.

T183-p - AMPKA1 (mouse)
Orthologous residues
AMPKA1 (human): T183‑p, AMPKA1 (mouse): T183‑p, AMPKA1 (rat): T183‑p, AMPKA1 (fruit fly): T184‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Comments:  AMPK alpha-2 KO mice
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
metformin increase
AICAR increase
MG132 AICAR increase
CK1E (human) decrease constitutively active
CK1E (human) increase domint negative
Downstream Regulation
 Effect of modification (function):  protein degradation
 Effect of modification (process):  transcription, altered
 Comments:  phaosphorylation induces degredation of mPer2 and expression of circadian clock genes.


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