Curated Information
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Curated Information Page
PubMed Id: 17438131 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Prickett TD, Brautigan DL (2007) Cytokine activation of p38 mitogen-activated protein kinase and apoptosis is opposed by alpha-4 targeting of protein phosphatase 2A for site-specific dephosphorylation of MEK3. Mol Cell Biol 27, 4217-27 17438131
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S218-p - MKK3 (human) ▲
Orthologous residues
MKK3 (human): S218‑p, MKK3 (mouse): S218‑p, MKK3 (rat): S290‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), 293T (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

T222-p - MKK3 (human) ▲
Orthologous residues
MKK3 (human): T222‑p, MKK3 (mouse): T222‑p, MKK3 (rat): T294‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), 293T (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
PHOSPHATASE PPP2CA (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP2CA (human) phospho-antibody, inhibition of upstream enzyme, antisense inhibition of upstream enzyme, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme
Downstream Regulation
 Effect of modification (function):  activation
 Effect of modification (process):  cell cycle regulation

T180-p - p38-alpha (human) ▲
Orthologous residues
p38‑alpha (human): T180‑p, p38‑alpha iso2 (human): T180‑p, p38‑alpha (mouse): T180‑p, p38‑alpha (rat): T180‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), 293T (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
anisomycin increase
IL-1b no change compared to control
sorbitol increase
Downstream Regulation
 Effect of modification (function):  activation
 Effect of modification (process):  cell cycle regulation

Y182-p - p38-alpha (human) ▲
Orthologous residues
p38‑alpha (human): Y182‑p, p38‑alpha iso2 (human): Y182‑p, p38‑alpha (mouse): Y182‑p, p38‑alpha (rat): Y182‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), 293T (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
anisomycin increase
IL-1b no change compared to control
sorbitol increase
Downstream Regulation
 Effect of modification (function):  activation
 Effect of modification (process):  cell cycle regulation


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