Curated Information
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Curated Information Page
PubMed Id: 18054859 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Samuels BA, et al. (2007) Cdk5 Promotes Synaptogenesis by Regulating the Subcellular Distribution of the MAGUK Family Member CASK. Neuron 56, 823-837 18054859
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S500-p - amphiphysin (mouse)
Orthologous residues
amphiphysin (human): S506‑p, amphiphysin (mouse): S500‑p, amphiphysin (rat): S496‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  neuron:synaptosome-brain
 Cellular systems studied:  tissue
 Species studied:  mouse

S51-p - CASK (mouse)
Orthologous residues
CASK (human): S51‑p, CASK iso2 (human): S51‑p, CASK iso3 (human): S51‑p, CASK (mouse): S51‑p, CASK iso3 (mouse): S51‑p, CASK (rat): S51‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial), CAD (neuron), COS (fibroblast), neuron:synaptosome-brain
 Cellular systems studied:  cell lines, tissue
 Species studied:  human, monkey, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK5 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK5 (mouse) pharmacological inhibitor of upstream enzyme, genetic knockout/knockin of upstream enzyme, transfection of dominant-negative enzyme, co-immunoprecipitation
Downstream Regulation
 Effect of modification (function):  intracellular localization, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
liprin alpha 1 (mouse) Disrupts pull-down assay
 Comments:  promotes CASK recruitment to presynaptic membranes and interaction with presynaptic proteins (Veli, Mint1, neurexins, N-type voltage-gated calcium channels); promotes calcium influx into neurons; promotes synaptogenesis;

Y113-p - CASK (mouse)
Orthologous residues
CASK (human): Y113‑p, CASK iso2 (human): Y113‑p, CASK iso3 (human): Y113‑p, CASK (mouse): Y113‑p, CASK iso3 (mouse): Y113‑p, CASK (rat): Y113‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

S119-p - CASK (mouse)
Orthologous residues
CASK (human): S119‑p, CASK iso2 (human): S119‑p, CASK iso3 (human): S119‑p, CASK (mouse): S119‑p, CASK iso3 (mouse): S119‑p, CASK (rat): S119‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

Y121-p - CASK (mouse)
Orthologous residues
CASK (human): Y121‑p, CASK iso2 (human): Y121‑p, CASK iso3 (human): Y121‑p, CASK (mouse): Y121‑p, CASK iso3 (mouse): Y121‑p, CASK (rat): Y121‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

S395-p - CASK (mouse)
Orthologous residues
CASK (human): S395‑p, CASK iso2 (human): S395‑p, CASK iso3 (human): S389‑p, CASK (mouse): S395‑p, CASK iso3 (mouse): S389‑p, CASK (rat): S395‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial), CAD (neuron), COS (fibroblast), neuron:synaptosome-brain
 Cellular systems studied:  cell lines, tissue
 Species studied:  human, monkey, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK5 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK5 (mouse) pharmacological inhibitor of upstream enzyme, genetic knockout/knockin of upstream enzyme, transfection of dominant-negative enzyme, co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
roscovitine decrease
Downstream Regulation
 Effect of modification (function):  intracellular localization, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
liprin alpha 1 (mouse) Disrupts pull-down assay
 Comments:  promotes CASK recruitment to presynaptic membranes and interaction with presynaptic proteins (Veli, Mint1, neurexins, N-type voltage-gated calcium channels); promotes calcium influx into neurons; promotes synaptogenesis;

S650-p - CASK (mouse)
Orthologous residues
CASK (human): S650‑p, CASK iso2 (human): S650‑p, CASK iso3 (human): S621‑p, CASK (mouse): S650‑p, CASK iso3 (mouse): S621‑p, CASK (rat): S638‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

Y708-p - CASK (mouse)
Orthologous residues
CASK (human): Y708‑p, CASK iso2 (human): Y708‑p, CASK iso3 (human): Y679‑p, CASK (mouse): Y708‑p, CASK iso3 (mouse): Y679‑p, CASK (rat): Y696‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

Y769-p - CASK (mouse)
Orthologous residues
CASK (human): Y769‑p, CASK iso2 (human): Y764‑p, CASK iso3 (human): Y740‑p, CASK (mouse): Y769‑p, CASK iso3 (mouse): Y740‑p, CASK (rat): Y752‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

T774-p - CASK (mouse)
Orthologous residues
CASK (human): T774‑p, CASK iso2 (human): T769‑p, CASK iso3 (human): T745‑p, CASK (mouse): T774‑p, CASK iso3 (mouse): T745‑p, CASK (rat): T757‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

T775-p - CASK (mouse)
Orthologous residues
CASK (human): T775‑p, CASK iso2 (human): T770‑p, CASK iso3 (human): T746‑p, CASK (mouse): T775‑p, CASK iso3 (mouse): T746‑p, CASK (rat): T758‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

S297-p - Doublecortin (mouse)
Orthologous residues
Doublecortin (human): S378‑p, Doublecortin iso2 (human): S297‑p, Doublecortin (mouse): S297‑p, Doublecortin iso3 (mouse): S297‑p, Doublecortin (rat): S297‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  neuron:synaptosome-brain
 Cellular systems studied:  tissue
 Species studied:  mouse

S1131-p - SYNJ1 (mouse)
Orthologous residues
SYNJ1 (human): S1134‑p, SYNJ1 iso3 (human): S1134‑p, SYNJ1 (mouse): S1131‑p, SYNJ1 iso3 (mouse): S1171‑p, SYNJ1 iso6 (mouse): S27‑p, SYNJ1 (rat): S1131‑p, SYNJ1 iso4 (rat): S1131‑p, SYNJ1 iso5 (rat): S1131‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  neuron:synaptosome-brain
 Cellular systems studied:  tissue
 Species studied:  mouse


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