Curated Information
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PubMed Id: 18054859 
Samuels BA, et al. (2007) Cdk5 Promotes Synaptogenesis by Regulating the Subcellular Distribution of the MAGUK Family Member CASK. Neuron 56, 823-837 18054859
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
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S500-p - amphiphysin (mouse)
Orthologous residues
amphiphysin (human): S506‑p, amphiphysin (mouse): S500‑p, amphiphysin (rat): S496‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  neuron:synaptosome-brain
 Cellular systems studied:  tissue
 Species studied:  mouse

S51-p - CASK (mouse)
Orthologous residues
CASK (human): S51‑p, CASK iso2 (human): S51‑p, CASK iso3 (human): S51‑p, CASK (mouse): S51‑p, CASK iso3 (mouse): S51‑p, CASK (rat): S51‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial), CAD (neuron), COS (fibroblast), neuron:synaptosome-brain
 Cellular systems studied:  cell lines, tissue
 Species studied:  human, monkey, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK5 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK5 (mouse) co-immunoprecipitation, pharmacological inhibitor of upstream enzyme, genetic knockout/knockin of upstream enzyme, transfection of dominant-negative enzyme
Downstream Regulation
 Effect of modification (function):  intracellular localization, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
liprin alpha 1 (mouse) Disrupts pull-down assay
 Comments:  promotes CASK recruitment to presynaptic membranes and interaction with presynaptic proteins (Veli, Mint1, neurexins, N-type voltage-gated calcium channels); promotes calcium influx into neurons; promotes synaptogenesis;

Y113-p - CASK (mouse)
Orthologous residues
CASK (human): Y113‑p, CASK iso2 (human): Y113‑p, CASK iso3 (human): Y113‑p, CASK (mouse): Y113‑p, CASK iso3 (mouse): Y113‑p, CASK (rat): Y113‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

S119-p - CASK (mouse)
Orthologous residues
CASK (human): S119‑p, CASK iso2 (human): S119‑p, CASK iso3 (human): S119‑p, CASK (mouse): S119‑p, CASK iso3 (mouse): S119‑p, CASK (rat): S119‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

Y121-p - CASK (mouse)
Orthologous residues
CASK (human): Y121‑p, CASK iso2 (human): Y121‑p, CASK iso3 (human): Y121‑p, CASK (mouse): Y121‑p, CASK iso3 (mouse): Y121‑p, CASK (rat): Y121‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

S395-p - CASK (mouse)
Orthologous residues
CASK (human): S395‑p, CASK iso2 (human): S395‑p, CASK iso3 (human): S389‑p, CASK (mouse): S395‑p, CASK iso3 (mouse): S389‑p, CASK (rat): S395‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial), CAD (neuron), COS (fibroblast), neuron:synaptosome-brain
 Cellular systems studied:  cell lines, tissue
 Species studied:  human, monkey, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK5 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK5 (mouse) co-immunoprecipitation, pharmacological inhibitor of upstream enzyme, genetic knockout/knockin of upstream enzyme, transfection of dominant-negative enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
roscovitine decrease
Downstream Regulation
 Effect of modification (function):  intracellular localization, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
liprin alpha 1 (mouse) Disrupts pull-down assay
 Comments:  promotes CASK recruitment to presynaptic membranes and interaction with presynaptic proteins (Veli, Mint1, neurexins, N-type voltage-gated calcium channels); promotes calcium influx into neurons; promotes synaptogenesis;

S650-p - CASK (mouse)
Orthologous residues
CASK (human): S650‑p, CASK iso2 (human): S650‑p, CASK iso3 (human): S621‑p, CASK (mouse): S650‑p, CASK iso3 (mouse): S621‑p, CASK (rat): S638‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

Y708-p - CASK (mouse)
Orthologous residues
CASK (human): Y708‑p, CASK iso2 (human): Y708‑p, CASK iso3 (human): Y679‑p, CASK (mouse): Y708‑p, CASK iso3 (mouse): Y679‑p, CASK (rat): Y696‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

Y769-p - CASK (mouse)
Orthologous residues
CASK (human): Y769‑p, CASK iso2 (human): Y764‑p, CASK iso3 (human): Y740‑p, CASK (mouse): Y769‑p, CASK iso3 (mouse): Y740‑p, CASK (rat): Y752‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

T774-p - CASK (mouse)
Orthologous residues
CASK (human): T774‑p, CASK iso2 (human): T769‑p, CASK iso3 (human): T745‑p, CASK (mouse): T774‑p, CASK iso3 (mouse): T745‑p, CASK (rat): T757‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

T775-p - CASK (mouse)
Orthologous residues
CASK (human): T775‑p, CASK iso2 (human): T770‑p, CASK iso3 (human): T746‑p, CASK (mouse): T775‑p, CASK iso3 (mouse): T746‑p, CASK (rat): T758‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

S297-p - Doublecortin (mouse)
Orthologous residues
Doublecortin (human): S378‑p, Doublecortin iso2 (human): S297‑p, Doublecortin (mouse): S297‑p, Doublecortin iso3 (mouse): S297‑p, Doublecortin (rat): S297‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  neuron:synaptosome-brain
 Cellular systems studied:  tissue
 Species studied:  mouse

S1131-p - SYNJ1 (mouse)
Orthologous residues
SYNJ1 (human): S1134‑p, SYNJ1 iso2 (human): S1134‑p, SYNJ1 iso3 (human): S1134‑p, SYNJ1 (mouse): S1131‑p, SYNJ1 iso3 (mouse): S1171‑p, SYNJ1 iso6 (mouse): S27‑p, SYNJ1 (rat): S1131‑p, SYNJ1 iso4 (rat): S1131‑p, SYNJ1 iso5 (rat): S1131‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  neuron:synaptosome-brain
 Cellular systems studied:  tissue
 Species studied:  mouse


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