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Orthologous residues
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|
IKK‑gamma (human): S68‑p, IKK‑gamma (mouse): S68‑p
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Characterization
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|
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling, mutation of modification site
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Relevant cell lines - cell types - tissues:
293 (epithelial)
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Cellular systems studied:
cell lines
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Species studied:
human
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Enzymes shown to modify site in vitro:
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|
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Upstream Regulation
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Treatments, proteins and their effect on site modification:
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Treatments
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Referenced Treatments
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Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
TNF
|
|
|
|
increase
|
|
|
okadaic acid
|
|
|
|
increase
|
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phorbol ester, ionomycin
|
|
|
|
no change compared to control
|
|
|
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Downstream Regulation
|
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Effect of modification (function):
molecular association, regulation
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
IKK-beta (human)
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Disrupts
|
|
|
co-immunoprecipitation
|
|
IKK-gamma (human)
|
|
Disrupts
|
|
|
chemical cross-linking
|
|
|
Comments:
phosphorylation attenuates amino-terminal protein dimerization; decreases TNF-alpha-induced NF-kB and IKKalpha activity.
|
