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Curated Information Page
PubMed Id: 19786542 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
McPherson VA, et al. (2009) SH2 domain-containing phosphatase-2 protein-tyrosine phosphatase promotes Fc(epsilon)RI-induced activation of Fyn and Erk pathways leading to TNF(alpha) release from bone marrow-derived mast cells. J Immunol 183, 4940-7 19786542
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S473-p - Akt1 (mouse)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  mast-bone marrow [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgE SHP-2 (mouse) increase
anti-IgE SHP-2 (mouse) increase SHP-2 KO decreases

T203-p - ERK1 (mouse)
Orthologous residues
ERK1 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  mast-bone marrow [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgE SHP-2 (mouse) increase
anti-IgE SHP-2 (mouse) increase SHP-2 KO decreases
anti-IgE SHP-2 (mouse) increase
anti-IgE SHP-2 (mouse) increase SHP-2 KO decreases

Y205-p - ERK1 (mouse)
Orthologous residues
ERK1 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  mast-bone marrow [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgE SHP-2 (mouse) increase
anti-IgE SHP-2 (mouse) increase SHP-2 KO decreases
anti-IgE SHP-2 (mouse) increase
anti-IgE SHP-2 (mouse) increase SHP-2 KO decreases

T183-p - ERK2 (mouse)
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  mast-bone marrow [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgE SHP-2 (mouse) increase
anti-IgE SHP-2 (mouse) increase SHP-2 KO decreases
anti-IgE SHP-2 (mouse) increase
anti-IgE SHP-2 (mouse) increase SHP-2 KO decreases

Y185-p - ERK2 (mouse)
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  mast-bone marrow [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgE SHP-2 (mouse) increase
anti-IgE SHP-2 (mouse) increase SHP-2 KO decreases
anti-IgE SHP-2 (mouse) increase
anti-IgE SHP-2 (mouse) increase SHP-2 KO decreases

Y531-p - Fyn (mouse)
Orthologous residues
Fyn (human): Y531‑p, Fyn iso2 (human): Y528‑p, Fyn iso3 (human): Y476‑p, Fyn (mouse): Y531‑p, Fyn (rat): Y531‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  mast-bone marrow [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgE SHP-2 (mouse) increase wild-type SHP-2
anti-IgE SHP-2 (mouse) no effect upon treatment-induced increase SHP-2 KO-no effect on treatment induced increase

Y441-p - Gab2 (mouse)
Orthologous residues
Gab2 (human): Y452‑p, Gab2 (mouse): Y441‑p, Gab2 (rat): Y441‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  mast-bone marrow [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgE SHP-2 (mouse) increase
anti-IgE SHP-2 (mouse) increase SHP-2 KO decreases

S180-p - IKKA (mouse)
Orthologous residues
IKKA (human): S180‑p, IKKA (mouse): S180‑p, IKKA (rat): S180‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  mast-bone marrow [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgE SHP-2 (mouse) increase
anti-IgE SHP-2 (mouse) increase SHP-2 KO decreases

S181-p - IKKB (mouse)
Orthologous residues
IKKB (human): S181‑p, IKKB (mouse): S181‑p, IKKB (rat): S181‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  mast-bone marrow [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgE SHP-2 (mouse) increase
anti-IgE SHP-2 (mouse) increase SHP-2 KO decreases

T183-p - JNK1 (mouse)
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  mast-bone marrow [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgE SHP-2 (mouse) increase wild-type SHP-2
anti-IgE SHP-2 (mouse) no effect upon treatment-induced increase SHP-2 KO-no effect on treatment induced increase

Y185-p - JNK1 (mouse)
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  mast-bone marrow [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgE SHP-2 (mouse) increase wild-type SHP-2
anti-IgE SHP-2 (mouse) no effect upon treatment-induced increase SHP-2 KO-no effect on treatment induced increase

Y397-p - Lyn (mouse)
Orthologous residues
Lyn (human): Y397‑p, Lyn iso2 (human): Y376‑p, Lyn (mouse): Y397‑p, Lyn iso2 (mouse): Y376‑p, Lyn (rat): Y397‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  mast-bone marrow [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgE SHP-2 (mouse) increase wild type SHP-2
anti-IgE SHP-2 (mouse) decrease SHP-2 KO increases

Y508-p - Lyn (mouse)
Orthologous residues
Lyn (human): Y508‑p, Lyn iso2 (human): Y487‑p, Lyn (mouse): Y508‑p, Lyn iso2 (mouse): Y487‑p, Lyn (rat): Y508‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  mast-bone marrow [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgE SHP-2 (mouse) increase wild-type
anti-IgE SHP-2 (mouse) increase SHP-2 KO decreases

T180-p - P38A (mouse)
Orthologous residues
P38A (human): T180‑p, P38A iso2 (human): T180‑p, P38A (mouse): T180‑p, P38A iso3 (mouse): T180‑p, P38A (rat): T180‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  mast-bone marrow [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgE SHP-2 (mouse) increase wild-type SHP-2
anti-IgE SHP-2 (mouse) no effect upon treatment-induced increase SHP-2 KO-no effect on treatment induced increase

Y182-p - P38A (mouse)
Orthologous residues
P38A (human): Y182‑p, P38A iso2 (human): Y182‑p, P38A (mouse): Y182‑p, P38A iso3 (mouse): Y182‑p, P38A (rat): Y182‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  mast-bone marrow [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgE SHP-2 (mouse) increase wild-type SHP-2
anti-IgE SHP-2 (mouse) no effect upon treatment-induced increase SHP-2 KO-no effect on treatment induced increase

Y783-p - PLCG1 (mouse)
Orthologous residues
PLCG1 (human): Y783‑p, PLCG1 iso2 (human): Y783‑p, PLCG1 (mouse): Y783‑p, PLCG1 (rat): Y783‑p, PLCG1 (cow): Y783‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  mast-bone marrow [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgE SHP-2 (mouse) increase wild-type SHP-2
anti-IgE SHP-2 (mouse) no effect upon treatment-induced increase SHP-2 KO-no effect on treatment induced increase

Y1217-p - PLCG2 (mouse)
Orthologous residues
PLCG2 (human): Y1217‑p, PLCG2 (mouse): Y1217‑p, PLCG2 (rat): Y1217‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  mast-bone marrow [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgE SHP-2 (mouse) increase wild type SHP-2
anti-IgE SHP-2 (mouse) decrease SHP-2 KO increases

Y346-p - Syk (mouse)
Orthologous residues
Syk (human): Y352‑p, Syk iso2 (human): Y329‑p, Syk (mouse): Y346‑p, Syk (rat): Y346‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  mast-bone marrow [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgE SHP-2 (mouse) increase wild-type SHP-2
anti-IgE SHP-2 (mouse) no effect upon treatment-induced increase SHP-2 KO-no effect on treatment induced increase


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