Curated Information
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Curated Information Page
PubMed Id: 11853558 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Sapkota GP, et al. (2002) Identification and characterization of four novel phosphorylation sites (Ser31, Ser325, Thr336 and Thr366) on LKB1/STK11, the protein kinase mutated in Peutz-Jeghers cancer syndrome. Biochem J 362, 481-90 11853558
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S31-p - LKB1 (mouse) ▲
Orthologous residues
LKB1 (human): S31‑p, LKB1 (mouse): S31‑p, LKB1 (rat): S31‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S325-p - LKB1 (mouse) ▲
Orthologous residues
LKB1 (human): S325‑p, LKB1 (mouse): S325‑p, LKB1 (rat): S325‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

T336-p - LKB1 (mouse) ▲
Orthologous residues
LKB1 (human): T336‑p, LKB1 (mouse): T336‑p, LKB1 (rat): T336‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phospho-antibody, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE LKB1 (mouse)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LKB1 (mouse) transfection of inactive enzyme, transfection of wild-type enzyme
Downstream Regulation
 Effect of modification (function):  inhibition
 Effect of modification (process):  cell growth, altered

T366-p - LKB1 (mouse) ▲
Orthologous residues
LKB1 (human): T363‑p, LKB1 (mouse): T366‑p, LKB1 (rat): T366‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phospho-antibody, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE LKB1 (mouse)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LKB1 (mouse) transfection of inactive enzyme, transfection of wild-type enzyme

S431-p - LKB1 (mouse) ▲
Orthologous residues
LKB1 (human): S428‑p, LKB1 (mouse): S431‑p, LKB1 (rat): S431‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human


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