|
Orthologous residues
|
|
PIAS1 (human): S90‑p, PIAS1 (mouse): S90‑p, PIAS1 (rat): S90‑p
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|
Characterization
|
|
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling, mass spectrometry, mutation of modification site, phospho-antibody
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|
Relevant cell lines - cell types - tissues:
293T (epithelial), macrophage-bone marrow, MEF (fibroblast) [IGF1R (mouse)], RAW 264 (macrophage), splenocyte
|
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Cellular systems studied:
cell lines, primary cultured cells
|
|
Species studied:
human, mouse
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Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
IKK-alpha (human)
|
siRNA inhibition of enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme
|
|
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
UV
|
|
|
|
increase
|
|
|
TNF
|
|
|
|
increase
|
|
|
LPS
|
|
|
|
increase
|
|
|
IFN-gamma
|
|
|
|
increase
|
|
|
anti-CD3/CD28
|
|
|
|
increase
|
|
|
|
Downstream Regulation
|
|
Effect of modification (process):
transcription, altered
|
|
Comments:
phosphorylation of this site inhibits TNF-induced expression of NFKB-target genes and IFN-induced expression of STAT1-target genes;
|
