Curated Information
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Curated Information Page
PubMed Id: 19833968 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Lamia KA, et al. (2009) AMPK regulates the circadian clock by cryptochrome phosphorylation and degradation. Science 326, 437-40 19833968
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S71-p - CRY1 (mouse)
Orthologous residues
CRY1 (human): S71‑p, CRY1 (mouse): S71‑p, CRY1 (rat): S71‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  AD293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AMPKA1 (human) pharmacological activator of upstream enzyme, activation of upstream enzyme, genetic knockout/knockin of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
AICAR increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, protein degradation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Per2 (human) Disrupts co-immunoprecipitation
FBXL3 (human) Induces co-immunoprecipitation
 Comments:  regulates circadian clock

S158-p - CRY1 (mouse)
Orthologous residues
CRY1 (human): S158‑p, CRY1 (mouse): S158‑p, CRY1 (rat): S158‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  AD293 (epithelial)
 Cellular systems studied:  cell lines

S247-p - CRY1 (mouse)
Orthologous residues
CRY1 (human): S247‑p, CRY1 (mouse): S247‑p, CRY1 (rat): S247‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  AD293 (epithelial)
 Cellular systems studied:  cell lines

T249-p - CRY1 (mouse)
Orthologous residues
CRY1 (human): T249‑p, CRY1 (mouse): T249‑p, CRY1 (rat): T249‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  AD293 (epithelial)
 Cellular systems studied:  cell lines

S280-p - CRY1 (mouse)
Orthologous residues
CRY1 (human): S280‑p, CRY1 (mouse): S280‑p, CRY1 (rat): S280‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  AD293 (epithelial), MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, protein degradation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
FBXL3 (human) Induces co-immunoprecipitation
Per2 (human) Induces co-immunoprecipitation
 Comments:  regulates circadian clock

S281-p - CRY1 (mouse)
Orthologous residues
CRY1 (human): S281‑p, CRY1 (mouse): S281‑p, CRY1 (rat): S281‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  AD293 (epithelial)
 Cellular systems studied:  cell lines

S575-p - CRY1 (mouse)
Orthologous residues
CRY1 (human): S555‑p, CRY1 (mouse): S575‑p, CRY1 (rat): S557‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  AD293 (epithelial)
 Cellular systems studied:  cell lines

S588-p - CRY1 (mouse)
Orthologous residues
CRY1 (human): S568‑p, CRY1 (mouse): S588‑p, CRY1 (rat): S570‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  AD293 (epithelial)
 Cellular systems studied:  cell lines

S595-p - CRY1 (mouse)
Orthologous residues
CRY1 (human): S575‑p, CRY1 (mouse): S595‑p, CRY1 (rat): S577‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  AD293 (epithelial)
 Cellular systems studied:  cell lines

S604-p - CRY1 (mouse)
Orthologous residues
CRY1 (human): S584‑p, CRY1 (mouse): S604‑p, CRY1 (rat): S586‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  AD293 (epithelial)
 Cellular systems studied:  cell lines


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