Curated Information
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Curated Information Page
PubMed Id: 19881501 
Smyth JT, et al. (2009) Phosphorylation of STIM1 underlies suppression of store-operated calcium entry during mitosis. Nat Cell Biol 11, 1465-72 19881501
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S486-p - STIM1 (human)
Orthologous residues
STIM1 (human): S486‑p, STIM1 (mouse): S486‑p, STIM1 (rat): S486‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
Downstream Regulation
 Effect of modification (function):  activity, inhibited
 Comments:  involved in suppression of store-operated calcium entry during mitosis

S492-p - STIM1 (human)
Orthologous residues
STIM1 (human): S492‑p, STIM1 (mouse): S492‑p, STIM1 (rat): S492‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S553-p - STIM1 (human)
Orthologous residues
STIM1 (human): S553‑p, STIM1 (mouse): S553‑p, STIM1 (rat): S553‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S575-p - STIM1 (human)
Orthologous residues
STIM1 (human): S575‑p, STIM1 (mouse): S575‑p, STIM1 (rat): S575‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole no change compared to control

S602-p - STIM1 (human)
Orthologous residues
STIM1 (human): S602‑p, STIM1 (mouse): S602‑p, STIM1 (rat): S602‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole decrease

S608-p - STIM1 (human)
Orthologous residues
STIM1 (human): S608‑p, STIM1 (mouse): S608‑p, STIM1 (rat): S608‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole decrease

S620-p - STIM1 (human)
Orthologous residues
STIM1 (human): S620‑p, STIM1 (mouse): S620‑p, STIM1 (rat): S620‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole no change compared to control

S621-p - STIM1 (human)
Orthologous residues
STIM1 (human): S621‑p, STIM1 (mouse): S621‑p, STIM1 (rat): S621‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole no change compared to control

S668-p - STIM1 (human)
Orthologous residues
STIM1 (human): S668‑p, STIM1 (mouse): S668‑p, STIM1 (rat): S668‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
Downstream Regulation
 Effect of modification (function):  activity, inhibited
 Comments:  involved in suppression of store-operated calcium entry during mitosis


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