Curated Information
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Curated Information Page
PubMed Id: 19881549 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Chan PC, Sudhakar JN, Lai CC, Chen HC (2010) Differential phosphorylation of the docking protein Gab1 by c-Src and the hepatocyte growth factor receptor regulates different aspects of cell functions. Oncogene 29, 698-710 19881549
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y48-p - Gab1 (human)
Orthologous residues
Gab1 (human): Y48‑p, Gab1 iso2 (human): Y48‑p, Gab1 (mouse): Y48‑p, Gab1 (rat): Y48‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

Y242-p - Gab1 (human)
Orthologous residues
Gab1 (human): Y242‑p, Gab1 iso2 (human): Y242‑p, Gab1 (mouse): Y242‑p, Gab1 (rat): Y241‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Src (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) transfection of wild-type enzyme
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CDK6 (human) Induces co-immunoprecipitation
 Comments:  promotes HGF-induced DNA synthesis

Y259-p - Gab1 (human)
Orthologous residues
Gab1 (human): Y259‑p, Gab1 iso2 (human): Y259‑p, Gab1 (mouse): Y259‑p, Gab1 (rat): Y258‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Src (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) transfection of wild-type enzyme
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CDK6 (human) Induces co-immunoprecipitation
 Comments:  promotes HGF-induced DNA synthesis

S266-p - Gab1 (human)
Orthologous residues
Gab1 (human): S266‑p, Gab1 iso2 (human): S266‑p, Gab1 (mouse): S266‑p, Gab1 (rat): S265‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry

Y307-p - Gab1 (human)
Orthologous residues
Gab1 (human): Y307‑p, Gab1 iso2 (human): Y307‑p, Gab1 (mouse): Y307‑p, Gab1 (rat): Y306‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

Y317-p - Gab1 (human)
Orthologous residues
Gab1 (human): Y317‑p, Gab1 iso2 (human): Y317‑p, Gab1 (mouse): Y317‑p, Gab1 (rat): Y316‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Src (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) transfection of wild-type enzyme
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CDK6 (human) Induces co-immunoprecipitation
 Comments:  promotes HGF-induced DNA synthesis

Y373-p - Gab1 (human)
Orthologous residues
Gab1 (human): Y373‑p, Gab1 iso2 (human): Y373‑p, Gab1 (mouse): Y373‑p, Gab1 (rat): Y372‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Src (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) transfection of wild-type enzyme
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CDK6 (human) Induces co-immunoprecipitation
 Comments:  promotes HGF-induced DNA synthesis

Y406-p - Gab1 (human)
Orthologous residues
Gab1 (human): Y406‑p, Gab1 iso2 (human): Y406‑p, Gab1 (mouse): Y407‑p, Gab1 (rat): Y405‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S419-p - Gab1 (human)
Orthologous residues
Gab1 (human): S419‑p, Gab1 iso2 (human): S419‑p, Gab1 (mouse): S420‑p, Gab1 (rat): S418‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry

Y447-p - Gab1 (human)
Orthologous residues
Gab1 (human): Y447‑p, Gab1 iso2 (human): Y447‑p, Gab1 (mouse): Y448‑p, Gab1 (rat): Y446‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

Y472-p - Gab1 (human)
Orthologous residues
Gab1 (human): Y472‑p, Gab1 iso2 (human): Y472‑p, Gab1 (mouse): Y473‑p, Gab1 (rat): Y471‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S551-p - Gab1 (human)
Orthologous residues
Gab1 (human): S551‑p, Gab1 iso2 (human): S581‑p, Gab1 (mouse): S552‑p, Gab1 (rat): S550‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry

Y589-p - Gab1 (human)
Orthologous residues
Gab1 (human): Y589‑p, Gab1 iso2 (human): Y619‑p, Gab1 (mouse): Y590‑p, Gab1 (rat): Y588‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

Y627-p - Gab1 (human)
Orthologous residues
Gab1 (human): Y627‑p, Gab1 iso2 (human): Y657‑p, Gab1 (mouse): Y628‑p, Gab1 (rat): Y626‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Src (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) transfection of wild-type enzyme

T638-p - Gab1 (human)
Orthologous residues
Gab1 (human): T638‑p, Gab1 iso2 (human): T668‑p, Gab1 (mouse): T639‑p, Gab1 (rat): T637‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry

Y659-p - Gab1 (human)
Orthologous residues
Gab1 (human): Y659‑p, Gab1 iso2 (human): Y689‑p, Gab1 (mouse): Y660‑p, Gab1 (rat): Y658‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human


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