Curated Information
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Curated Information Page
PubMed Id: 19720745 
Dibble CC, Asara JM, Manning BD (2009) Characterization of Rictor phosphorylation sites reveals direct regulation of mTOR complex 2 by S6K1. Mol Cell Biol 29, 5657-70 19720745
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S21-p - Rictor (human)
Orthologous residues
Rictor (human): S21‑p, Rictor (mouse): S21‑p, Rictor (rat): S6‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S35-p - Rictor (human)
Orthologous residues
Rictor (human): S35‑p, Rictor (mouse): S35‑p, Rictor (rat): S20‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S265-p - Rictor (human)
Orthologous residues
Rictor (human): S265‑p, Rictor (mouse): S265‑p, Rictor (rat): S250‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

T1103-p - Rictor (human)
Orthologous residues
Rictor (human): T1103‑p, Rictor (mouse): T1103‑p, Rictor (rat): T1088‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

T1135-p - Rictor (human)
Orthologous residues
Rictor (human): T1135‑p, Rictor (mouse): T1135‑p, Rictor (rat): T1120‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  liver cancer
 Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3-L1 (fibroblast), C2C12 (myoblast), HepG2 (hepatic)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE p70S6K (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE p70S6K (human) siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
rapamycin insulin inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, phosphorylation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces co-immunoprecipitation, pull-down assay
 Comments:  cells expressing Rictor T1135A exhibit an increase in insulin-stimulated Akt-S473 and TSC2-T1462 phosphorylation

S1138-p - Rictor (human)
Orthologous residues
Rictor (human): S1138‑p, Rictor (mouse): S1138‑p, Rictor (rat): S1123‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S1219-p - Rictor (human)
Orthologous residues
Rictor (human): S1219‑p, Rictor (mouse): S1218‑p, Rictor (rat): S1204‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S1235-p - Rictor (human)
Orthologous residues
Rictor (human): S1235‑p, Rictor (mouse): S1234‑p, Rictor (rat): S1220‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

T1271-p - Rictor (human)
Orthologous residues
Rictor (human): T1271‑p, Rictor (mouse): T1270‑p, Rictor (rat): T1256‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S1274-p - Rictor (human)
Orthologous residues
Rictor (human): S1274‑p, Rictor (mouse): S1273‑p, Rictor (rat): A1259‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S1302-p - Rictor (human)
Orthologous residues
Rictor (human): S1302‑p, Rictor (mouse): S1301‑p, Rictor (rat): S1287‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S1313-p - Rictor (human)
Orthologous residues
Rictor (human): S1313‑p, Rictor (mouse): S1312‑p, Rictor (rat): S1298‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S1346-p - Rictor (human)
Orthologous residues
Rictor (human): S1346‑p, Rictor (mouse): S1345‑p, Rictor (rat): S1331‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S1353-p - Rictor (human)
Orthologous residues
Rictor (human): S1353‑p, Rictor (mouse): S1352‑p, Rictor (rat): S1338‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

T1376-p - Rictor (human)
Orthologous residues
Rictor (human): T1376‑p, Rictor (mouse): T1375‑p, Rictor (rat): T1361‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

Y1386-p - Rictor (human)
Orthologous residues
Rictor (human): Y1386‑p, Rictor (mouse): Y1385‑p, Rictor (rat): Y1371‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S1388-p - Rictor (human)
Orthologous residues
Rictor (human): S1388‑p, Rictor (mouse): S1387‑p, Rictor (rat): S1373‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S1396-p - Rictor (human)
Orthologous residues
Rictor (human): S1396‑p, Rictor (mouse): S1395‑p, Rictor (rat): S1381‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S1571-p - Rictor (human)
Orthologous residues
Rictor (human): S1571‑p, Rictor (mouse): S1570‑p, Rictor (rat): S1556‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S1574-p - Rictor (human)
Orthologous residues
Rictor (human): S1574‑p, Rictor (mouse): S1573‑p, Rictor (rat): S1559‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S1577-p - Rictor (human)
Orthologous residues
Rictor (human): S1577‑p, Rictor (mouse): S1576‑p, Rictor (rat): S1562‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S473-p - Akt1 (mouse)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin Rictor (human) augment treatment-induced increase Rictor T1135A mutant

T1465-p - TSC2 (mouse)
Orthologous residues
TSC2 (human): T1462‑p, TSC2 iso3 (human): T1418‑p, TSC2 iso4 (human): T1439‑p, TSC2 (mouse): T1465‑p, TSC2 iso6 (mouse): T1443‑p, TSC2 (rat): T1466‑p, TSC2 iso2 (rat): T1423‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin Rictor (human) augment treatment-induced increase Rictor T1135A mutant


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