Curated Information
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Curated Information Page
PubMed Id: 19834457 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Taniguchi S, et al. (2009) Involvement of NMDAR2A tyrosine phosphorylation in depression-related behaviour. EMBO J 28, 3717-29 19834457
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y943-p - NMDAR2A (human) ▲
Orthologous residues
NMDAR2A (human): Y943‑p, NMDAR2A (mouse): Y943‑p, NMDAR2A (rat): Y943‑p
Characterization
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Fyn (human)

Y1105-p - NMDAR2A (human) ▲
Orthologous residues
NMDAR2A (human): Y1105‑p, NMDAR2A (mouse): Y1105‑p, NMDAR2A (rat): Y1105‑p
Characterization
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Fyn (human)

Y1118-p - NMDAR2A (human) ▲
Orthologous residues
NMDAR2A (human): Y1118‑p, NMDAR2A (mouse): Y1118‑p, NMDAR2A (rat): Y1118‑p
Characterization
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Fyn (human)

Y1187-p - NMDAR2A (human) ▲
Orthologous residues
NMDAR2A (human): Y1187‑p, NMDAR2A (mouse): Y1187‑p, NMDAR2A (rat): Y1187‑p
Characterization
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Fyn (human)

Y1246-p - NMDAR2A (human) ▲
Orthologous residues
NMDAR2A (human): Y1246‑p, NMDAR2A (mouse): Y1246‑p, NMDAR2A (rat): Y1246‑p
Characterization
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Fyn (human)

Y1267-p - NMDAR2A (human) ▲
Orthologous residues
NMDAR2A (human): Y1267‑p, NMDAR2A (mouse): Y1267‑p, NMDAR2A (rat): Y1267‑p
Characterization
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Fyn (human)

Y1325-p - NMDAR2A (human) ▲
Orthologous residues
NMDAR2A (human): Y1325‑p, NMDAR2A (mouse): Y1325‑p, NMDAR2A (rat): Y1325‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Fyn (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) transfection of constitutively active enzyme
Downstream Regulation
 Effect of modification (function):  activation

T34-p - DARPP-32 (mouse) ▲
Orthologous residues
DARPP‑32 (human): T34‑p, DARPP‑32 (mouse): T34‑p, DARPP‑32 (rat): T34‑p, DARPP‑32 (cow): T34‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'brain, striatum'
 Cellular systems studied:  tissue
 Species studied:  mouse
 Comments:  NMDAR2A Y1325F/Y1325F mutant mice
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NMDAR2A (mouse) decrease increases in NMDAR2A Y1325F/Y1325F mutant mice
Associated Diseases
Diseases: Alterations: Comments:
depression mutation of site

S896-p - NMDAR1 (mouse) ▲
Orthologous residues
NMDAR1 (human): S896‑p, NMDAR1 iso2 (human): ‑, NMDAR1 iso5 (human): S917‑p, NMDAR1 (mouse): S896‑p, NMDAR1 iso2 (mouse): ‑, NMDAR1 iso3 (mouse): S917‑p, NMDAR1 (rat): S896‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'brain, striatum'
 Cellular systems studied:  tissue
 Species studied:  mouse
 Comments:  NMDAR2A Y1325F/Y1325F mutant mice
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
forced swim test no change compared to control

Y1325-p - NMDAR2A (mouse) ▲
Orthologous residues
NMDAR2A (human): Y1325‑p, NMDAR2A (mouse): Y1325‑p, NMDAR2A (rat): Y1325‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'brain, striatum'
 Cellular systems studied:  tissue
 Species studied:  mouse
 Comments:  NMDAR2A Y1325F/Y1325F mutant mice
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
forced swim test increase
tail suspension test increase
open field test no change compared to control
elevated plus maze test no change compared to control
Downstream Regulation
 Effect of modification (function):  activation, phosphorylation
 Comments:  mutation of this site increases phosphorylation of T34 of DARPP-32
Associated Diseases
Diseases: Alterations: Comments:
depression mutation of site

Y1472-p - NMDAR2B (mouse) ▲
Orthologous residues
NMDAR2B (human): Y1474‑p, NMDAR2B (mouse): Y1472‑p, NMDAR2B (rat): Y1472‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'brain, striatum'
 Cellular systems studied:  tissue
 Species studied:  mouse
 Comments:  NMDAR2A Y1325F/Y1325F mutant mice
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
forced swim test no change compared to control

Y424-p - Src (mouse) ▲
Orthologous residues
Src (human): Y419‑p, Src (mouse): Y424‑p, Src iso2 (mouse): Y418‑p, Src (rat): Y419‑p, Src (chicken): Y416‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'brain, striatum'
 Cellular systems studied:  tissue
 Species studied:  mouse
 Comments:  NMDAR2A Y1325F/Y1325F mutant mice
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
forced swim test increase


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