Curated Information
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Curated Information Page
PubMed Id: 19861535 
Zhi G, et al. (2009) Fanconi anemia complementation group FANCD2 protein serine 331 phosphorylation is important for fanconi anemia pathway function and BRCA2 interaction. Cancer Res 69, 8775-83 19861535
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
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S331-p - FANCD2 (human)
Orthologous residues
FANCD2 (human): S331‑p, FANCD2 iso1 (human): S331‑p, FANCD2 iso3 (human): S331‑p, FANCD2 (mouse): S329‑p, FANCD2 (rat): S331‑p, FANCD2 (chicken): S334‑p
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  Fanconi's anaemia
 Relevant cell lines - cell types - tissues:  BD180 (lymphoblast), EUFA143, EUFA868, GM6914 (fibroblast), HeLa (cervical), HSC72, PD20
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Chk1 (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
mitomycin C increase
SB218078 mitomycin C inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, ubiquitination
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BRCA2 (human) Induces co-immunoprecipitation
 Comments:  confers resistance to mitomycin C

K561-ub - FANCD2 (human)
Orthologous residues
FANCD2 (human): K561‑ub, FANCD2 iso1 (human): K561‑ub, FANCD2 iso3 (human): K561‑ub, FANCD2 (mouse): K559‑ub, FANCD2 (rat): K561‑ub, FANCD2 (chicken): K563‑ub
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  PD20
 Cellular systems studied:  cell lines
 Species studied:  human

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