Curated Information
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Curated Information Page
PubMed Id: 19861535 
Zhi G, et al. (2009) Fanconi anemia complementation group FANCD2 protein serine 331 phosphorylation is important for fanconi anemia pathway function and BRCA2 interaction. Cancer Res 69, 8775-83 19861535
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S331-p - FANCD2 (human)
Orthologous residues
FANCD2 (human): S331‑p, FANCD2 iso2 (human): S331‑p, FANCD2 (mouse): S329‑p, FANCD2 (rat): S331‑p, FANCD2 (chicken): S334‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  Fanconi's anaemia
 Relevant cell lines - cell types - tissues:  BD180 (lymphoblast), EUFA143, EUFA868, GM6914 (fibroblast), HeLa (cervical), HSC72, PD20
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Chk1 (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
mitomycin C increase
SB218078 mitomycin C inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, ubiquitination
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BRCA2 (human) Induces co-immunoprecipitation
 Comments:  confers resistance to mitomycin C

K561-ub - FANCD2 (human)
Orthologous residues
FANCD2 (human): K561‑ub, FANCD2 iso2 (human): K561‑ub, FANCD2 (mouse): K559‑ub, FANCD2 (rat): K561‑ub, FANCD2 (chicken): K563‑ub
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  PD20
 Cellular systems studied:  cell lines
 Species studied:  human


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