Curated Information
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Curated Information Page
PubMed Id: 10025949 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Chen ZP, et al. (1999) AMP-activated protein kinase phosphorylation of endothelial NO synthase. FEBS Lett 443, 285-9 10025949
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T494-p - eNOS (rat)
Orthologous residues
eNOS (human): T495‑p, eNOS (mouse): T494‑p, eNOS (rat): T494‑p, eNOS (rabbit): T501‑p, eNOS (pig): T497‑p, eNOS (cow): T497‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  heart
 Cellular systems studied:  tissue
 Species studied:  rat
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE AMPKA1 (rat)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AMPKA1 (rat) co-immunoprecipitation
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited
 Comments:  Phosphorylated In the absence of Ca2+ inhibiting eNOS, but not during ischaemia.

S1176-p - eNOS (rat)
Orthologous residues
eNOS (human): S1177‑p, eNOS (mouse): S1176‑p, eNOS (rat): S1176‑p, eNOS (rabbit): S1183‑p, eNOS (pig): S1179‑p, eNOS (cow): S1179‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  heart
 Cellular systems studied:  tissue
 Species studied:  rat
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE AMPKA1 (rat)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AMPKA1 (rat) co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ischemia increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Comments:  Activated in vitro and during ischaemia in the presence of Ca2+ calmodulin.


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