Curated Information
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Curated Information Page
PubMed Id: 19789182 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Tifft KE, Bradbury KA, Wilson KL (2009) Tyrosine phosphorylation of nuclear-membrane protein emerin by Src, Abl and other kinases. J Cell Sci 122, 3780-90 19789182
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

Y4-p - emerin (human) ▲
Orthologous residues
emerin (human): Y4‑p, emerin (mouse): Y4‑p, emerin (rat): Y4‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BAF (human) Disrupts in vitro

Y19-p - emerin (human) ▲
Orthologous residues
emerin (human): Y19‑p, emerin (mouse): Y19‑p, emerin (rat): Y19‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Abl (human)
KINASE Src (human)
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BAF (human) Induces in vitro
BAF (human) Induces co-immunoprecipitation

Y34-p - emerin (human) ▲
Orthologous residues
emerin (human): Y34‑p, emerin (mouse): Y34‑p, emerin (rat): Y34‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BAF (human) Induces co-immunoprecipitation
BAF (human) Induces in vitro

Y41-p - emerin (human) ▲
Orthologous residues
emerin (human): Y41‑p, emerin (mouse): Y41‑p, emerin (rat): Y41‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Abl (human)
KINASE Src (human)

Y59-p - emerin (human) ▲
Orthologous residues
emerin (human): Y59‑p, emerin (mouse): Y60‑p, emerin (rat): Y60‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical) [Src (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Src (human)
KINASE Abl (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) transfection of inactive enzyme, transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
vanadate increase

Y74-p - emerin (human) ▲
Orthologous residues
emerin (human): Y74‑p, emerin (mouse): Y75‑p, emerin (rat): Y75‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical) [Src (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Src (human)
KINASE Abl (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) transfection of inactive enzyme, transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
vanadate increase

Y85-p - emerin (human) ▲
Orthologous residues
emerin (human): Y85‑p, emerin (mouse): Y86‑p, emerin (rat): Y86‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Src (human)
KINASE Abl (human)

Y90-p - emerin (human) ▲
Orthologous residues
emerin (human): Y90‑p, emerin (mouse): Y91‑p, emerin (rat): Y91‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Src (human)
KINASE Abl (human)

Y94-p - emerin (human) ▲
Orthologous residues
emerin (human): Y94‑p, emerin (mouse): Y95‑p, emerin (rat): Y95‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Abl (human)
KINASE Src (human)

Y95-p - emerin (human) ▲
Orthologous residues
emerin (human): Y95‑p, emerin (mouse): Y96‑p, emerin (rat): Y96‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical) [Src (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Abl (human)
KINASE Src (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) transfection of inactive enzyme, transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
vanadate increase

Y99-p - emerin (human) ▲
Orthologous residues
emerin (human): Y99‑p, emerin (mouse): Y100‑p, emerin (rat): Y100‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Src (human)
KINASE Abl (human)

Y105-p - emerin (human) ▲
Orthologous residues
emerin (human): Y105‑p, emerin (mouse): Y106‑p, emerin (rat): Y106‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Src (human)
KINASE Abl (human)

Y155-p - emerin (human) ▲
Orthologous residues
emerin (human): Y155‑p, emerin (mouse): Y155‑p, emerin (rat): Y156‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Abl (human)

Y161-p - emerin (human) ▲
Orthologous residues
emerin (human): Y161‑p, emerin (mouse): Y161‑p, emerin (rat): Y162‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Src (human)
KINASE Abl (human)
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BAF (human) Induces in vitro
BAF (human) Induces co-immunoprecipitation
BAF (human) Induces in vitro

Y167-p - emerin (human) ▲
Orthologous residues
emerin (human): Y167‑p, emerin (mouse): Y167‑p, emerin (rat): Y168‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Src (human)
KINASE Abl (human)

Y181-p - emerin (human) ▲
Orthologous residues
emerin (human): Y181‑p, emerin (mouse): Y182‑p, emerin (rat): Y183‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Src (human)

Y182-p - emerin (human) ▲
Orthologous residues
emerin (human): Y182‑p, emerin (mouse): Y183‑p, emerin (rat): Y184‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Src (human)


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