Curated Information
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Curated Information Page
PubMed Id: 9748248 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Huang C, Liu J, Haudenschild CC, Zhan X (1998) The role of tyrosine phosphorylation of cortactin in the locomotion of endothelial cells. J Biol Chem 273, 25770-6 9748248
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y421-p - cortactin (human) ▲
Orthologous residues
cortactin (human): Y421‑p, cortactin iso3 (human): Y384‑p, cortactin (mouse): Y421‑p, cortactin (rat): Y383‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, peptide sequencing, phospho-antibody, phosphoamino acid analysis
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [cortactin (human)], 3T3 (fibroblast) [Src (human)]
 Cellular systems studied:  cell lines
 Species studied:  mouse
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Comments:  Previous work by the authors showed that src attenuates the actin cross-linking activity of cortactin. These studies show that transfection with a cortactin mutant (F421/F470/F486) impairs the motility of endothelial cells.

Y470-p - cortactin (human) ▲
Orthologous residues
cortactin (human): Y470‑p, cortactin iso3 (human): Y433‑p, cortactin (mouse): Y466‑p, cortactin (rat): Y428‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, peptide sequencing, phospho-antibody, phosphoamino acid analysis
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [cortactin (human)], 3T3 (fibroblast) [Src (human)]
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Src (human)
 Comments:  It appears that v-src may have been used for in vitro and well as in in vivo studies.
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Comments:  Previous work by the authors showed that src attenuates the actin cross-linking activity of cortactin. These studies show that transfection with a cortactin mutant (F421/F470/F486) impairs the motility of endothelial cells.

Y486-p - cortactin (human) ▲
Orthologous residues
cortactin (human): Y486‑p, cortactin iso3 (human): Y449‑p, cortactin (mouse): Y482‑p, cortactin (rat): Y444‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, peptide sequencing, phospho-antibody, phosphoamino acid analysis
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [cortactin (human)], 3T3 (fibroblast) [Src (human)]
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Src (human)
 Comments:  It appears that v-src may have been used for in vitro and well as in in vivo studies.
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Comments:  Previous work by the authors showed that src attenuates the actin cross-linking activity of cortactin. These studies show that transfection with a cortactin mutant (F421/F470/F486) impairs the motility of endothelial cells.


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