Curated Information
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Curated Information Page
PubMed Id: 19801631 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Wang X, She H, Mao Z (2009) Phosphorylation of neuronal survival factor MEF2D by glycogen synthase kinase 3beta in neuronal apoptosis. J Biol Chem 284, 32619-26 19801631
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S9-p - GSK3B (rat)
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S3‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  neuron-'brain, cerebellum'
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
KCl decrease low concentration
lithium KCl inhibit treatment-induced decrease
kenpaullone KCl inhibit treatment-induced decrease
insulin KCl inhibit treatment-induced decrease
valproic acid KCl inhibit treatment-induced decrease
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited

Y216-p - GSK3B (rat)
Orthologous residues
GSK3B (human): Y216‑p, GSK3B iso2 (human): Y216‑p, GSK3B (mouse): Y216‑p, GSK3B (rat): Y216‑p, GSK3B (rabbit):
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  neuron-'brain, cerebellum'
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
KCl increase low concentration

T145-p - MEF2D (rat)
Orthologous residues
MEF2D (human): T145‑p, MEF2D (mouse): T145‑p, MEF2D iso2 (mouse): T152‑p, MEF2D (rat): T145‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  neuron-'brain, cerebellum'
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3B (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) transfection of inactive enzyme, modification site within consensus motif
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
KCl increase low concentration
Downstream Regulation
 Effect of modification (function):  activity, inhibited
 Effect of modification (process):  apoptosis, altered, transcription, inhibited

S149-p - MEF2D (rat)
Orthologous residues
MEF2D (human): S149‑p, MEF2D (mouse): S149‑p, MEF2D iso2 (mouse): S156‑p, MEF2D (rat): S149‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  neuron-'brain, cerebellum'
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3B (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) transfection of inactive enzyme, modification site within consensus motif
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
KCl increase low concentration
Downstream Regulation
 Effect of modification (function):  activity, inhibited
 Effect of modification (process):  apoptosis, altered, transcription, inhibited

S153-p - MEF2D (rat)
Orthologous residues
MEF2D (human): S153‑p, MEF2D (mouse): S153‑p, MEF2D iso2 (mouse): S160‑p, MEF2D (rat): S153‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  neuron-'brain, cerebellum'
 Cellular systems studied:  primary cultured cells
 Species studied:  rat
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3B (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) transfection of inactive enzyme, modification site within consensus motif
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
KCl increase low concentration
Downstream Regulation
 Effect of modification (function):  activity, inhibited
 Effect of modification (process):  apoptosis, altered, transcription, inhibited


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