Curated Information
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PubMed Id: 12556497 
Ishitani T, Ninomiya-Tsuji J, Matsumoto K (2003) Regulation of lymphoid enhancer factor 1/T-cell factor by mitogen-activated protein kinase-related Nemo-like kinase-dependent phosphorylation in Wnt/beta-catenin signaling. Mol Cell Biol 23, 1379-89 12556497
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T155-p - LEF-1 (human)
Orthologous residues
LEF‑1 (human): T155‑p, LEF‑1 iso6 (human): T155‑p, LEF‑1 (mouse): T153‑p, LEF‑1 iso3 (mouse): T153‑p, LEF‑1 (rat): T153‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE NLK (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE NLK (human) transfection of wild-type enzyme, mutation in upstream enzyme recognition motif
Downstream Regulation
 Effect of modification (process):  transcription, inhibited

S166-p - LEF-1 (human)
Orthologous residues
LEF‑1 (human): S166‑p, LEF‑1 iso6 (human): S166‑p, LEF‑1 (mouse): S164‑p, LEF‑1 iso3 (mouse): S164‑p, LEF‑1 (rat): S164‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE NLK (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE NLK (human) transfection of wild-type enzyme, mutation in upstream enzyme recognition motif
Downstream Regulation
 Effect of modification (process):  transcription, inhibited

T201-p - TCF7L2 (human)
Orthologous residues
TCF7L2 (human): T201‑p, TCF7L2 iso4 (human): T201‑p, TCF7L2 iso10 (human): T178‑p, TCF7L2 (mouse): T178‑p, TCF7L2 (rat): T201‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE NLK (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE NLK (human) transfection of wild-type enzyme, mutation in upstream enzyme recognition motif
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DNA Disrupts
 Comments:  Beta-catenin-TCF7L2 complex DNA binding

T212-p - TCF7L2 (human)
Orthologous residues
TCF7L2 (human): T212‑p, TCF7L2 iso4 (human): T212‑p, TCF7L2 iso10 (human): T189‑p, TCF7L2 (mouse): T189‑p, TCF7L2 (rat): T212‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE NLK (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE NLK (human) transfection of wild-type enzyme, mutation in upstream enzyme recognition motif
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DNA Disrupts
 Comments:  Beta-catenin-TCF7L2 complex DNA binding


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